Vio® Dye antibody conjugates

Brighter dyes for flow cytometry

  • Bright mean fluorescence intensity for excellent signals
  • High stain index for clear population discrimination
  • Hassle-free multicolor experiments due to low compensation

Vio and VioBright Dyes represent a family of fluorochromes for flow cytometry and fluorescence microscopy. They are characterized by high fluorescence intensities and low spillover, making them an ideal choice for multicolor applications.

Combined with traditional fluorochromes, such as FITC, PE, PerCP, and APC, they expand available dye options, giving you more flexibility in setting up multiparameter cell analyses 

Excitation and emission data of Vio Dyes

FluorochromeExcitationExmax (nm)Emmax (nm)MACSQuant AnalyzerMACSQuant VYB
laser (nm)ChannelFilter (nm)ChannelFilter (nm)
VioBlue405400452V1450/50V1450/50
VioGreen405388520V2525/50V2525/50
VioBright 515488488514B1525/50B1525/50
Vio 515488488514B1525/50B1525/50
VioBright FITC488496522B1525/50B1525/50
FITC488495520B1525/50B1525/50
PE488 or 561565578B2585/40Y1586/15
PE-Vio 615488 or 561565619B3655-730Y2615/20
PerCP488482675B3655-730N/AN/A
PerCP-Vio 700488482704B3655-730N/AN/A
PE-Vio 770488 or 561565775B4750 LPY4750 LP
APC561 or 635652660R1655-730Y3661/20
APC-Vio 770561 or 635652775R2750 LPY4750 LP
Vio 667             561 or 635645668R1655–730Y3661/20
Vio Bright 667  561 or 635645668R1655–730Y3661/20

Violet laser (405 nm)

VioBlue® Dye (Emmax 452 nm)

Designed to maximize the potential of a flow cytometer’s violet laser, the VioBlue® Dye shows superior performance compared to many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioBlue exhibits minimal photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.

VioBlue Dye at a glance:

  • Coumarin-based dye with excitation and emission wavelengths of 400 nm and 455 nm, respectively
  • Brighter alternative to Pacific Blue™, Alexa Fluor® 405, or BD Horizon™ V450
  • Multiplexing of VioBlue with other fluorochromes is easily possible, adding to the variety of marker combination for multiparameter flow cytometry
  • Combined use with the VioGreen Dye

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission maxima of fluorochromes related to VioBlue.
FluorochromeAbsorption max. (nm)Emission max. (nm)
VioBlue    400455
Pacific Blue405455
 Cascade Blue(375); 401423
Alexa Fluor 405405421
eFluor 405405450
BD Horizon V450404448

VioGreen™ Dye (Emmax 520 nm)

Designed to maximize the potential of a flow cytometer’s violet laser, the VioGreen™ Dye shows superior performance compared with many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioGreen exhibits minimal levels of photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.

VioGreen Dye at a glance:

  • Large Stokes shift fluorophore, emitting strong fluorescence at 520 nm upon excitation at 405 nm
  • Significantly increased mean fluorescence intensities and higher stain indices than Pacific Orange™, Krome Orange™, and BD Horizon™ V500
  • Non-protein fluorophore
  • Combined use with the VioBlue Dye
  • Perfectly suited for multiparameter flow cytometry using all current flow cytometer

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission maxima of fluorochromes related to VioGreen.
FluorochromeAbsorption max. (nm)Emission max. (nm)
VioGreen388520
Pacific Orange400551
Krome Orange398528
AmCyan458489
HorizonV500415500

Blue laser (488nm) 

VioBright™ 515 and Vio 515 (Emmax 514 nm)

VioBright 515 and Vio 515 are extremely bright dyes for the B1/FITC-channel. The proprietary VioBright multimerization technology results in a 4-fold increase in brightness over the traditional FITC dye. With a 25% reduced spill-over into the B2/PE-channel and high photo stability, these dyes are excellent choices for immunofluorescence microscopy applications.


VioBright 515 at a glance:

  • Brightest Vio Dye for the blue laser
  • Excellent choice for surface markers, in particular for low expressed antigens

Vio 515 at a glance:

  • Brighter alternative to FITC
  • Perfectly suited for intracellular markers, such as cytokines and transcription factors

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

VioBright™ FITC (Emmax 522 nm)

VioBright FITC is a blue laser (488 nm) excited revolutionary dye, which allows an increased number of FITC molecules per antibody, as compared to conventional FITC conjugation. With brightness similar to PE, VioBright FITC expands the dimensions of multicolor flow analysis. In addition, it provides a bright alternative for confident detection of rare cells, as well as dim and uncharacterized markers.

VioBright FITC at a glance:

  • Brightness similar to PE for confident detection of low-expressed and rare markers
  • Excellent bright alternative to PE for flexible multicolor panel design
  • Signal detectable in standard FITC channel with peak excitation and emission wavelength of 496 nm and 522 nm, respectively
  • Works with your standard staining protocol

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission peaks of fluorochromes compared to VioBright 515, Vio 515, and VioBright FITC.
FluorochromeAbsorption max. (nm)Emission max. (nm)
VioBright 515488514
Vio 515488514
VioBright FITC496522
FITC495520
Alexa Fluor 488495519
BD Horizon Brilliant Blue 515490515

PerCP-Vio 700 (Emmax 704 nm)

The PerCP-Vio 700 Dye is a tandem conjugate that combines the peridinin chlorophyll protein (PerCP) and the new Vio 700 Dye to emit a strong fluorescence at 655–730 nm upon blue laser excitation at 488 nm.  This dye is suited perfectly for the B3 channel of the MACSQuant® Analyzer.

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission maximums of fluorochromes comparable to PerCP-Vio 700.
FluorochromeAbsorption max. (nm)Emission max. (nm)
PerCP482675
PerCP-Vio 700482704
PerCP-Cy5.5490695

Blue laser (488 nm)/yellow laser (561 nm) 

PE-Vio 770 (Emmax 770 nm)

The PE-Vio 770 Dye is a tandem conjugate, like PE-Cy™7, that exploits the principle of fluorescence-resonance-energy-transfer (FRET) allowing for large Stokes shifts between the absorbed energy of a fluorescence donor (PE) and the emission wavelength of a suitable acceptor (Vio 770) dye.

PE-Vio 770 Dye at a glance:
  • Excitation with the blue (488 nm) or yellow (561 nm) laser, emission in the near-infrared region at 775 nm.
  • The combination of Vio 770 as the acceptor dye and an optimized chemistry have furnished a tandem dye that is characterized by a high fluorescence intensity, minimal spillover to adjacent detection channels, and low non-specific binding to non-target cells to meet the complexity of multiparameter flow cytometry.
  • Higher MFI and stain index values, in addition to lower compensation settings when compared to PE-Cy7

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission maximums of fluorochromes comparable to PE-Vio 770.
FluorochromeAbsorption max. (nm)Emission max. (nm)
PE-Vio 770
565775
PE-Cy7496774
PE-Alexa Fluor 750
496775

PE-Vio 615 (Emmax 619 nm)

PE-Vio 615 is a tandem dye with PE as the donor dye and Vio 615 as the acceptor dye. This tandem dye is optimized for efficient donor-to-acceptor dye energy transfer, high fluorescent intensity, and low spillover into the donor dye detection channel. Designed to be a superior alternative to ECD, PE-Texas Red®, PE-efluor® 610, PE-CF594 and PE/Dazzle™ 594, this Vio Dye expands the options for flexible multicolor panel design and provides a bright dye for confident detection of dim and rare markers.

PE-Vio 615 at a glance:
  • Optimal excitation with blue (488 nm), green (532 nm), and yellow green (561 nm) laser lines for maximum flexibility. 
  • Excellent brightness for confident detection of dim, rare, and uncharacterized markers.
  • Extensive portfolio of PE-Vio 615 dye conjugated to recombinantly engineered REAfinity™ Antibodies for higher reproducibility.

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission maximums of fluorochromes comparable to PE-Vio 615.
FluorochromeAbsorption max. (nm)Emission max. (nm)
PE-Vio 615565619
ECD, PE-Texas Red565613
PE-efluor 610565606
PE-CF594565614
PE/Dazzle 594566612

Red laser (635 nm) 

APC-Vio 770 (Emmax 770 nm)

The APC-Vio 770 Dye is a tandem conjugate, like APC-Cy™7 or APC-H7, that exploits the principle of fluorescence-resonance-energy transfer (FRET) allowing for large Stokes shifts between the absorbed energy of a fluorescence donor (APC) and the emission wavelength of a suitable acceptor (Vio 770) dye.

APC-Vio 770 Dye at a glance:
  • Excitation with the yellow (561 nm) or red (635 nm) laser, emission in the near-infrared region at 775 nm.
  • The combination of Vio 770 as the acceptor dye and an optimized chemistry have furnished a tandem dye that is characterized by a high fluorescence intensity, minimal spillover to adjacent detection channels, and low non-specific binding to non-target cells to meet the complexity of multiparameter flow cytometry.
  • Higher MFI and stain index values, in addition to lower compensation settings when compared to APC-Cy7.

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission maxima of fluorochromes related to APC-Vio 770.
FluorochromeAbsorption max. (nm)Emission max. (nm)
APC-Vio 770625775
APC-Cy7650774
APC-Alexa Fluor750775

VioBright 667 and Vio 667 (Emmax 668 nm)

VioBright 667 and Vio 667 are extremely bright dyes for the R1/APC-channel. The proprietary VioBright multimerization technology results in a 2-fold increase in brightness over the traditional APC dye. 

VioBright 667 at a glance:

  • Brighter alternative to APC
  • Brightest Vio Dye for the red laser
  • Excellent choice for surface markers, in particular for low expressed antigens

VioBright 667 at a glance:

  • As bright as APC
  • Perfectly suited for intracellular markers, such as cytokines and transcription factors

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

Absorption and emission maxima of fluorochromes related to APC-Vio 770.
FluorochromeAbsorption max. (nm)Emission max. (nm)
VioBright 667645668
Vio 667645668
APC496522

Supporting material

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