CD94 antibodies, human

CD94 antibodies, human

Clone: REA113 | Dilution: 1:50
The clone REA113 recognizes CD94, an approximately 45 kDa, single-pass type II transmembrane protein, which belongs to the C-type lectin superfamily. CD94 associates covalently with NKG2 family members and forms disulfide bonded heterodimers. CD94-NKG2 receptors bind HLA-E, the non-classical MHC class Ib molecule. Depending on the associated NKG2 member, CD94-NKG2 heterodimer transmits either activating or inhibitory signal. For, e.g, via immunoreceptor tyrosine based-inhibitory motifs (ITIMs) containing NKG2A, CD94-NKG2A heterodimers inhibit NK cell cytotoxicity. Besides NK cells, expression of CD94-NKG2 heterodimers is also shown on NK-T cells and a small percentage of CD8 cells in mice, where the crosslinking of CD94-NKG2 was shown to reduce the level of spontaneous apoptosis.
Additional information: Clone REA113 displays negligible binding to Fc receptors.

Alternative Names

KLRD1, Kp43

Technical specifications

  • Antigen: CD94
  • Clone: REA113
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: KLRD1, Kp43
  • Entrez Gene ID: 3824
  • Molecular mass of antigen [kDa]: 21
  • Distribution of antigen: NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PerCP-Vio700
  • Selected References

    1. Gunturi, A. et al. (2003) Preferential survival of CD8 T and NK cells expressing high levels of CD94. J. Immunol. 170: 1737-1745
    2. Braud, V. M. et al. (1998) HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C. Nature 391(6669): 795-799
    3. Brooks, A. G. et al. (1997) NKG2A complexed with CD94 defines a novel inhibitory natural killer cell receptor. J. Exp. Med. 185: 795-800
    4. Jeffery, H. et al. Human intrahepatic ILC2 are IL-13positive amphiregulinpositive and their frequency correlates with model of end stage liver disease score. PLoS One 12(12)
    5. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
    6. Canté-Barrett, K. et al. (2017)
      Loss of CD44
      dim
      expression from early progenitor cells marks T-cell lineage commitment in the human thymus.
      Front Immunol 8: 32

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA113
HP-3D9++
DX22++
Product options: 2

Product information

Size
Order no.
Price

CD94-PerCP-Vio700, human

  • REA113
  • 1:50
  • For research use only
for 30
tests
(1)
130-119-803
EUR 120,00 

CD94-PerCP-Vio700, human

  • REA113
  • 1:50
  • For research use only
for 100
tests
(1)
130-119-763
EUR 290,00