CD94 antibodies, human

CD94 antibodies, human

Clone: REA113 | Dilution: 1:11
The clone REA113 recognizes CD94, an approximately 45 kDa, single-pass type II transmembrane protein, which belongs to the C-type lectin superfamily. CD94 associates covalently with NKG2 family members and forms disulfide bonded heterodimers. CD94-NKG2 receptors bind HLA-E, the non-classical MHC class Ib molecule. Depending on the associated NKG2 member, CD94-NKG2 heterodimer transmits either activating or inhibitory signal. For, e.g, via immunoreceptor tyrosine based-inhibitory motifs (ITIMs) containing NKG2A, CD94-NKG2A heterodimers inhibit NK cell cytotoxicity. Besides NK cells, expression of CD94-NKG2 heterodimers is also shown on NK-T cells and a small percentage of CD8 cells in mice, where the crosslinking of CD94-NKG2 was shown to reduce the level of spontaneous apoptosis.
Additional information: Clone REA113 displays negligible binding to Fc receptors.

Alternative Names

KLRD1, Kp43

Technical specifications

  • Antigen: CD94
  • Clone: REA113
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: KLRD1, Kp43
  • Entrez Gene ID: 3824
  • Molecular mass of antigen [kDa]: 21
  • Distribution of antigen: NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA113 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA113
HP-3D9++
DX22++
  • Selected references

    1. Gunturi, A. et al. (2003) Preferential survival of CD8 T and NK cells expressing high levels of CD94. J. Immunol. 170: 1737-1745
    2. Braud, V. M. et al. (1998) HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C. Nature 391(6669): 795-799
    3. Brooks, A. G. et al. (1997) NKG2A complexed with CD94 defines a novel inhibitory natural killer cell receptor. J. Exp. Med. 185: 795-800
    4. Jeffery, H. et al. Human intrahepatic ILC2 are IL-13positive amphiregulinpositive and their frequency correlates with model of end stage liver disease score. PLoS One 12(12)
    5. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
    6. Canté-Barrett, K. et al. (2017)
      Loss of CD44
      dim
      expression from early progenitor cells marks T-cell lineage commitment in the human thymus.
      Front Immunol 8: 32
Product options: 12
130-098-975

CD94-FITC, human

for 30
tests
(1)
EUR 90,00 
130-098-971

CD94-FITC, human

for 100
tests
(1)
EUR 220,00 
130-098-973

CD94-PE, human

for 30
tests
(1)
EUR 110,00 
130-098-974

CD94-PE, human

for 100
tests
(1)
EUR 265,00 
130-098-978

CD94-APC, human

for 30
tests
(1)
EUR 110,00 
130-098-976

CD94-APC, human

for 100
tests
(1)
EUR 265,00 
130-098-980

CD94-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-098-979

CD94-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-101-172

CD94-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-101-146

CD94-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-098-967

CD94-Biotin, human

for 30
tests
(1)
EUR 90,00 
130-098-966

CD94-Biotin, human

for 100
tests
(1)
EUR 220,00