Find the products and resources you are looking for!
Miltenyi Biotec distribution:
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
For immediate technical support, use our live chat. Connect with us
Technical support - Clinical products
CD14+ monocytes were isolated with CD14 MicroBeads, human, from peripheral blood mononuclear cells (PBMCs) and cultured in the presence of GM-CSF and IL-4 for 6 days into inmature monocyte-derived dendritic cells (Mo-DCs). For generation of mature MoDCs, cells were cultured for another 24 hours in the presence of TNF-a, IL-6 and IL-1β. MoDCs, either inmature (left image) or mature were stained with CD83 antibodies as well as with CD14 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.