CD73 antibodies, human

CD73 antibodies, human

Clone: REA804 | Dilution: 1:50
Clone REA804 recognizes the human CD73 antigen, which is a 70 kDa membrane-bound enzyme that catalyzes the conversion of AMP to bioactive adenosine at neutral pH. This glycoprotein is also called ecto-5’-nucleotidase. CD73 is present on subsets of T and B lymphocytes, follicular dendritic cells, endothelial cells, and marrow stromal cells (MSCs).
Additional information: Clone REA804 displays negligible binding to Fc receptors.

Alternative Names

NT5E, CALJA, E5NT, NT, Nt5, NTE, eN, eNT

Technical specifications

  • Antigen: CD73
  • Clone: REA804
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: NT5E, CALJA, E5NT, NT, Nt5, NTE, eN, eNT
  • Entrez Gene ID: 4907
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    chimpanzee (
    Pan troglodytes
    )
  • Distribution of antigen: B cells, dendritic cells, endothelial cells, epithelial cells, lymphocytes, mesenchymal stem cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio615, PE-Vio770, VioBright 515, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA804 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA804
AD2++
  • Selected references

    1. Thomson, L. F. et al. (1990) Production and characterization of monoclonal antibodies to the glycosyl Tissue Antigens 35: 9-19
    2. Hansen, K. R. et al. (1995) Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene. Gene 167(1-2): 307-312
    3. Pittenger, M. F. et al. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147
Product options: 12
130-112-060

CD73-PE, human

for 30
tests
(1)
EUR 110,00 
130-111-908

CD73-PE, human

for 100
tests
(1)
EUR 265,00 
130-112-061

CD73-APC, human

for 30
tests
(1)
EUR 110,00 
130-111-909

CD73-APC, human

for 100
tests
(1)
EUR 265,00 
130-112-064

CD73-PE-Vio615, human

for 30
tests
(1)
EUR 120,00 
130-111-912

CD73-PE-Vio615, human

for 100
tests
(1)
EUR 290,00 
130-112-062

CD73-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-111-910

CD73-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-112-065

CD73-VioBright 515, human

for 30
tests
(1)
EUR 150,00 
130-111-913

CD73-VioBright 515, human

for 100
tests
(1)
EUR 360,00 
130-112-058

CD73-Biotin, human

for 30
tests
(1)
EUR 90,00 
130-111-906

CD73-Biotin, human

for 100
tests
(1)
EUR 220,00