CD65 antibodies, human

CD65 antibodies, human

Clone: VIM8 | Dilution: 1:11
Clone VIM8 recognizes the human CD65 antigen, a fucosylated carbohydrate on the cell surface, which is also known as ceramide dodecasaccharide or VIM-2. CD65 is formed by fucosylation of the inner lactosamine unit of α-2,3-sialylated polylactosamine by α-1,3-fucosyltransferase. It is expressed on all myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood. The sialylated form of CD65 (designated CD65s) appears when the progenitor antigen CD34 disappears, suggesting that this sialylated carbohydrate antigen marks a turning point in normal myeloid differentiation. CD65 is a critical adhesion molecule for extravascular acute myeloid leukemia cell infiltration.

Alternative names

VIM-2

Technical specifications

  • Antigen: CD65
  • Clone: VIM8
  • Isotype: mouse IgM
  • Isotype control: Mouse IgM – isotype control antibodies
  • Alternative names of antigen: VIM-2
  • Distribution of antigen: granulocytes, macrophages, monocytes, myeloid stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, APC-Vio770, PerCP-Vio700
  • Selected references

    1. Lund-Johansen, F. et al. (1992) Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65). J. Immunol. 148(10): 3221-3229
    2. Bordessoule, D. et al. (1993) Immunohistological patterns of myeloid antigens: tissue distribution of CD13, CD14, CD16, CD31, CD36, CD65, CD66 and CD67. Br. J. Haematol. 83(3): 370-383
    3. Kniep, B. et al. (1996)
      The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V
      3
      FucnLc
      8
      Cer.
      J. Biochem. 119(3): 456-462
Product options: 10
130-108-262

CD65-FITC, human

for 30
tests
(1)
EUR 90,00 
130-108-235

CD65-FITC, human

for 100
tests
(1)
EUR 220,00 
130-108-263

CD65-PE, human

for 30
tests
(1)
EUR 110,00 
130-108-236

CD65-PE, human

for 100
tests
(1)
EUR 265,00 
130-108-264

CD65-APC, human

for 30
tests
(1)
EUR 110,00 
130-108-237

CD65-APC, human

for 100
tests
(1)
EUR 265,00 
130-108-266

CD65-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-108-239

CD65-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-108-267

CD65-PerCP-Vio700, human

for 30
tests
(1)
EUR 120,00 
130-108-240

CD65-PerCP-Vio700, human

for 100
tests
(1)
EUR 290,00