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Mouse bEnd.3 cells were either left unstimulated (middle peak) or stimulated with 20 ng/mL TNF-α for 5 hours. Cells were then stained with CD62E antibodies (right peak) or with the corresponding REA Control (S) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.