CD56 antibodies, human

CD56 antibodies, human

Clone: REA196 | Dilution: 1:11
Clone REA196 recognizes the human CD56 antigen, a glycoprotein of the Ig-superfamily, also known as neural cell adhesion molecule (NCAM), which is expressed in blood on practically all resting and activated NK cells and on a minor subset of CD3
+
T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal-derived tumors, myelomas, and myeloid leukemias also express CD56.
Additional information: Clone REA196 displays negligible binding to Fc receptors.

Alternative Names

NCAM1, MSK39, NCAM

Technical specifications

  • Antigen: CD56
  • Clone: REA196
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: NCAM1, MSK39, NCAM
  • Entrez Gene ID: 4684
  • Molecular mass of antigen [kDa]: 92
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    olive baboon (
    Papio anubis
    )
  • Distribution of antigen: NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE-Vio615, VioBright 667
  • Selected References

    1. Camp, B. V. et al. (1990) Plasma cells in multiple myeloma express a natural killer cell- associated antigen: CD56 (NKH-1; Leu-19). Blood 76: 377-382
    2. Boin, F. et al. (2017) Flow cytometric discrimination of seven lineage markers by using two fluorochromes. PLoS One 12(11)
    3. Petty, R. D. et al. (2016) MicroRNAs hsa-miR-99b, hsa-miR-330, hsa-miR-126 and hsa-miR-30c: potential diagnostic biomarkers in natural killer (NK) cells of patients with chronic fatigue syndrome (CFS)/ myalgic encephalomyelitis (ME). PLoS One 11: e0150904
    4. Dong, P. et al. (2017) Simultaneous detection of decidual Th1/Th2 and NK1/NK2 immunophenotyping in unknown recurrent miscarriage using 8-color flow cytometry with FSC/Vt extended strategy. Biosci. Rep. 37(3)
    5. Canté-Barrett, K. et al. (2017)
      Loss of CD44
      dim
      expression from early progenitor cells marks T-cell lineage commitment in the human thymus.
      Front Immunol 8: 32
    6. Delso-Vallejo, M. et al. (2017) Influence of Irradiated Peripheral Blood Mononuclear Cells on Both Ex Vivo Proliferation of Human Natural Killer Cells and Change in Cellular Property. Front Immunol 8
    7. Veluchamy, J. et al. (2017)
      In vivo
      efficacy of umbilical cord blood stem cell-derived NK cells in the treatment of metastatic colorectal cancer.
      Front Immunol 8: 87
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clones Overlap in epitope recognition with REA196
NCAM16.2 ++
TULY56 ++
5.1H11 ++
AF12-7H3 -
C5.9 ++
N901/NKH1 ++
CMSSB +
B159

 -

HCD56-
MEM188-
Product options: 3

Product information

Size
Order no.
Price

CD56-PE-Vio615, human

  • REA196
  • 1:11
  • For research use only
for 30
tests
(1)
130-107-193
EUR 120,00 

CD56-VioBright 667, human

  • REA196
  • 1:11
  • For research use only
for 30
tests
(1)
130-111-926
EUR 150,00 

CD56-VioBright 667, human

  • REA196
  • 1:11
  • For research use only
for 100
tests
(1)
130-111-755
EUR 360,00 

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