CD54 (ICAM-1) antibodies, mouse

CD54 (ICAM-1) antibodies, mouse

Clone: REA171 | Dilution: 1:10
Clone REA171 recognizes the murine CD54 antigen, a type I transmembrane glycoprotein also known as intercellular adhesion molecule 1 (ICAM-1). CD54 is continuously present in low concentrations in the membranes of monocytes/macrophages, lymphocytes, activated endothelial cells, granulocytes, and dendritic cells. Its expression is regulated by inflammatory cytokines and can be induced by interleukin-1 and tumor necrosis factor. CD54 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). When activated, leukocytes bind to endothelial cells via CD54/LFA-1 and then transmigrate into tissues.
Additional information: Clone REA171 displays negligible binding to Fc receptors.

Alternative Names

ICAM-1, Ly-47, MALA-2

Technical specifications

  • Antigen: CD54 (ICAM-1)
  • Clone: REA171
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: ICAM-1, Ly-47, MALA-2
  • Entrez Gene ID: 15894
  • Molecular mass of antigen [kDa]: 56
  • Distribution of antigen: granulocytes, lymphocytes, macrophages, monocytes, endothelial cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, VioBright 515, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA171
3E2-
YN1/174++
KAT-1+
  • Selected References

    1. Fine, J. S. et al. (1991) The role of LFA-1/ICAM-1 interactions during murine T lymphocyte development. J. Immunol. 147(9): 2852-2859
    2. Siu, G. et al. (1989) Isolation of the murine intercellular adhesion molecule 1 (ICAM-1) gene. ICAM-1 enhances antigen-specific T cell activation. J. Immunol. 143(11): 3813-3820
    3. Driessens, M. H. et al. (1996) Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct. J. Leukoc. Biol. 60(6): 758-765
Product options: 14

Product information

Size
Order no.
Price

CD54 (ICAM-1)-FITC, mouse

  • REA171
  • 1:10
  • For research use only
9 µg in 300 µL
130-104-246
EUR 20,00 

CD54 (ICAM-1)-FITC, mouse

  • REA171
  • 1:10
  • For research use only
30 µg in 1 mL
130-104-214
EUR 40,00 

CD54 (ICAM-1)-PE, mouse

  • REA171
  • 1:10
  • For research use only
9 µg in 300 µL
130-104-247
EUR 27,00 

CD54 (ICAM-1)-PE, mouse

  • REA171
  • 1:10
  • For research use only
30 µg in 1 mL
130-104-215
EUR 80,00 

CD54 (ICAM-1)-APC, mouse

  • REA171
  • 1:10
  • For research use only
9 µg in 300 µL
130-104-248
EUR 44,00 

CD54 (ICAM-1)-APC, mouse

  • REA171
  • 1:10
  • For research use only
30 µg in 1 mL
130-104-216
EUR 80,00 

CD54 (ICAM-1)-PE-Vio770, mouse

  • REA171
  • 1:10
  • For research use only
9 µg in 300 µL
130-104-249
EUR 44,00 

CD54 (ICAM-1)-PE-Vio770, mouse

  • REA171
  • 1:10
  • For research use only
30 µg in 1 mL
130-104-217
EUR 100,00 

CD54 (ICAM-1)-APC-Vio770, mouse

  • REA171
  • 1:10
  • For research use only
9 µg in 300 µL
130-104-250
EUR 54,00 

CD54 (ICAM-1)-APC-Vio770, mouse

  • REA171
  • 1:10
  • For research use only
30 µg in 1 mL
130-104-218
EUR 100,00 

CD54 (ICAM-1)-VioBright 515, mouse

  • REA171
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-109-048
EUR 71,00 

CD54 (ICAM-1)-VioBright 515, mouse

  • REA171
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-108-992
EUR 145,00 

CD54 (ICAM-1)-Biotin, mouse

  • REA171
  • 1:10
  • For research use only
9 µg in 300 µL
130-104-245
EUR 15,00 

CD54 (ICAM-1)-Biotin, mouse

  • REA171
  • 1:10
  • For research use only
30 µg in 1 mL
130-104-213
EUR 40,00