CD45RA antibodies, human

CD45RA antibodies, human

Clone: REA562 | Dilution: 1:50
Clone REA562 recognizes the human CD45RA antigen, which is expressed on naive CD4
+
and CD8
+
T cells as well as on CD8
+
effector T cells. CD45RA is also present on subsets of B and NK cells as well as on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA), a transmembrane tyrosine phosphatase.
Additional information: Clone REA562 displays negligible binding to Fc receptors

Alternative names

Ptprc, B220, CD45R, GP180, L-CA, LY5, T200

Technical specifications

  • Antigen: CD45RA
  • Clone: REA562
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Ptprc, B220, CD45R, GP180, L-CA, LY5, T200
  • Entrez Gene ID: 5788
  • Molecular mass of antigen [kDa]: 145
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: T cells, B cells, NK cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA562 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA562
HI100-
L48++
JS-83++
T6D11++
REA1047-
  • Selected references

    1. Braun, T. et al. (2012)
      Effector CD4
      +
      CD45RA
      CD25
      bright
      Foxp3
      bright
      regulatory T cell (eTreg) distribution is significantly impaired in chronic myelomonocytic leukemia (CMML) and correlates with TET 2 mutational status.
      Blood 120: 2808-2808
    2. Baba, N. et al. (2010)
      Interplay between CD45RA
      +
      regulatory T cells and TNF-α in the regulation of human Tʜ17 differentiation.
      Int. Immunol. 22: 237-244
    3. Cantisan, S. et al. (2010)
      CD45RA expression on HCMV-specific effector memory CD8
      +
      T cells is associated with the duration and intensity of HCMV replication after transplantation.
      Clin. Immunol. 137(1): 81-88
Product options: 18
130-113-927

CD45RA-FITC, human

for 30
tests
(1)
EUR 70,00 
130-113-365

CD45RA-FITC, human

for 100
tests
(1)
EUR 175,00 
130-113-928

CD45RA-PE, human

for 30
tests
(1)
EUR 95,00 
130-113-366

CD45RA-PE, human

for 100
tests
(1)
EUR 235,00 
130-113-924

CD45RA-APC, human

for 30
tests
(1)
EUR 95,00 
130-113-362

CD45RA-APC, human

for 100
tests
(1)
EUR 235,00 
130-113-931

CD45RA-VioGreen, human

for 30
tests
(1)
EUR 115,00 
130-113-369

CD45RA-VioGreen, human

for 100
tests
(1)
EUR 275,00 
130-118-847

CD45RA-PE-Vio615, human

for 30
tests
(1)
EUR 120,00 
130-118-789

CD45RA-PE-Vio615, human

for 100
tests
(1)
EUR 290,00 
130-113-929

CD45RA-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-113-367

CD45RA-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-113-925

CD45RA-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-113-363

CD45RA-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-113-930

CD45RA-PerCP-Vio700, human

for 30
tests
(1)
EUR 120,00 
130-113-368

CD45RA-PerCP-Vio700, human

for 100
tests
(1)
EUR 290,00 
130-113-926

CD45RA-Biotin, human

for 30
tests
(1)
EUR 70,00 
130-113-364

CD45RA-Biotin, human

for 100
tests
(1)
EUR 175,00