CD45 antibodies, mouse

CD45 antibodies, mouse

Clone: REA737 | Dilution: 1:50
Clone REA737 recognizes the mouse CD45 antigen, also known as Ly-5 or leukocyte common antigen (LCA), which belongs to the protein tyrosine phosphatase (PTP) family. CD45 is a glycoprotein which is involved in T cell receptor and B cell receptor signal transduction. It is expressed at high levels on all cells of hematopoietic origin except for erythrocytes. Clone REA737 reacts with all CD45 isoforms. CD45 can be used to discriminate leukocytes from non-hematopoietic cells.
Additional information: Clone REA737 displays negligible binding to Fc receptors.

Alternative names

L-CA, LCA, Ly-5, T200

Technical specifications

  • Antigen: CD45
  • Clone: REA737
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: L-CA, LCA, Ly-5, T200
  • Entrez Gene ID: 19264
  • Molecular mass of antigen [kDa]: 142
  • Distribution of antigen: T cells, B cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA737 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA737
30F11++
1312.3+
IBL-5/25++
  • Selected references

    1. Pulido, R. et al. (1988) Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities. J. Immunol. 140(11): 3851-3857
    2. Arendt, C. W. et al. (1997) Identification of the CD45-associated 116 kDa and 80 kDa proteins as the alpha- and beta-subunits of alpha-glucosidase II. J. Biol. Chem. 272(20): 13117-13125
    3. Trowbridge, I. S. et al. (1994) CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu. Rev. Immunol. 12: 85-116
Product options: 20
130-110-796

CD45-FITC, mouse

30 µg in 200 µL (1)
EUR 35,00 
130-110-658

CD45-FITC, mouse

4×150 µg in 1 mL (1)
EUR 185,00 
130-110-797

CD45-PE, mouse

30 µg in 200 µL (1)
EUR 60,00 
130-110-659

CD45-PE, mouse

2×150 µg in 1 mL (1)
EUR 210,00 
130-110-798

CD45-APC, mouse

30 µg in 200 µL (1)
EUR 60,00 
130-110-660

CD45-APC, mouse

150 µg in 1 mL (1)
EUR 175,00 
130-110-802

CD45-VioBlue, mouse

30 µg in 200 µL (1)
EUR 95,00 
130-110-664

CD45-VioBlue, mouse

150 µg in 1 mL (1)
EUR 275,00 
130-110-803

CD45-VioGreen, mouse

30 µg in 200 µL (1)
EUR 95,00 
130-110-665

CD45-VioGreen, mouse

150 µg in 1 mL (1)
EUR 275,00 
130-110-804

CD45-PE-Vio615, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-110-666

CD45-PE-Vio615, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-110-799

CD45-PE-Vio770, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-110-661

CD45-PE-Vio770, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-110-800

CD45-APC-Vio770, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-110-662

CD45-APC-Vio770, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-110-801

CD45-PerCP-Vio700, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-110-663

CD45-PerCP-Vio700, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-110-795

CD45-Biotin, mouse

30 µg in 200 µL (1)
EUR 35,00 
130-110-657

CD45-Biotin, mouse

4×150 µg in 1 mL (1)
EUR 185,00