CD45 antibodies, human

CD45 antibodies, human

Clone: REA747 | Dilution: 1:50
Clone REA747 recognizes the human CD45 antigen, a member of the protein tyrosine phosphatase (PTP) family, also known as the leukocyte common antigen (LCA). The CD45 molecule is a type I transmembrane protein which is required for T and B cell activation and involved in cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 is expressed in different isoforms (CD45RA, CD45RB, CD45RC, CD45RAB, CD45RAC, CD45RBC, CD45RO, CD45R (ABC)) depending on the differentiation status of the cell. The CD45 antibody recognizes a common epitope of all CD45 isoforms.
Additional information: Clone REA747 displays negligible binding to Fc receptors.

Alternative Names

Ptprc, GP180, L-CA, LY5, T200

Technical specifications

  • Antigen: CD45
  • Clone: REA747
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Ptprc, GP180, L-CA, LY5, T200
  • Entrez Gene ID: 5788
  • Molecular mass of antigen [kDa]: 145
  • Distribution of antigen: B cells, basophils, dendritic cells, granulocytes, hematopoietic stem cells, Langerhans cells, leukocytes, lymphocytes, macrophages, mast cells, monocytes, plasma cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, VioBright 667, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA747 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA747
2D1++
HI30++
5B1++
MB4-6D6-
REA1023-
  • Selected References

    1. Ralph, S. J. et al. (1987) Structural variants of human T200 glycoprotein (leukocyte-common antigen). EMBO J. 6(5): 1251-1257
    2. Vykoukal, J. et al. (2008) Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation. Lab Chip 8(8): 1386-1393
    3. Kurian, L. et al. (2013) Conversion of human fibroblasts to angioblast-like progenitor cells. Nat. Methods 10(1): 77-83
Product options: 25
130-110-769

CD45-FITC, human

for 30
tests
(1)
EUR 70,00 
130-110-631

CD45-FITC, human

for 100
tests
(1)
EUR 175,00 
130-114-569

CD45-FITC, human

for 500
tests
(1)
EUR 440,00 
130-110-770

CD45-PE, human

for 30
tests
(1)
EUR 95,00 
130-110-632

CD45-PE, human

for 100
tests
(1)
EUR 235,00 
130-110-771

CD45-APC, human

for 30
tests
(1)
EUR 95,00 
130-110-633

CD45-APC, human

for 100
tests
(1)
EUR 235,00 
130-110-775

CD45-VioBlue, human

for 30
tests
(1)
EUR 115,00 
130-110-637

CD45-VioBlue, human

for 100
tests
(1)
EUR 275,00 
130-110-776

CD45-VioGreen, human

for 30
tests
(1)
EUR 115,00 
130-110-638

CD45-VioGreen, human

for 100
tests
(1)
EUR 275,00 
130-110-777

CD45-PE-Vio615, human

for 30
tests
(1)
EUR 120,00 
130-110-639

CD45-PE-Vio615, human

for 100
tests
(1)
EUR 290,00 
130-110-772

CD45-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-110-634

CD45-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-110-773

CD45-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-110-635

CD45-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-110-774

CD45-PerCP-Vio700, human

for 30
tests
(1)
EUR 120,00 
130-110-636

CD45-PerCP-Vio700, human

for 100
tests
(1)
EUR 290,00 
130-110-778

CD45-VioBright 515, human

for 30
tests
(1)
EUR 150,00 
130-110-640

CD45-VioBright 515, human

for 100
tests
(1)
EUR 360,00 
130-110-779

CD45-VioBright 667, human

for 30
tests
(1)
EUR 150,00 
130-110-641

CD45-VioBright 667, human

for 100
tests
(1)
EUR 360,00 
130-110-768

CD45-Biotin, human

for 30
tests
(1)
EUR 70,00 
130-110-630

CD45-Biotin, human

for 100
tests
(1)
EUR 175,00