CD4 antibodies, mouse

CD4 antibodies, mouse

Clone: REA604 | Dilution: 1:10
Clone REA604 recognizes the mouse CD4 antigen, a cell surface glycoprotein, which is a member of the immunoglobulin superfamily. It is also known as L3T4 or Leu-3. In mice, CD4 is expressed on T helper cells, regulatory T cells, and at lower levels on subpopulations of NKT cells and dendritic cells. It is furthermore detected on most thymocytes (CD4
+
CD8
+
and CD4
+
CD8
thymocytes). CD4 interacts with MHC class II molecules on the surface of antigen-presenting cells. It increases the avidity of the interaction between T cells and antigen-presenting or target cells.
Additional information: Clone REA604 displays negligible binding to Fc receptors.

Alternative names

L3T4, T4, Leu-3, Ly-4

Technical specifications

  • Antigen: CD4
  • Clone: REA604
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: L3T4, T4, Leu-3, Ly-4
  • Entrez Gene ID: 12504
  • Molecular mass of antigen [kDa]: 48
  • Distribution of antigen: T cells, NKT cells, dendritic cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE-Vio770, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA604 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA604
GK1.5++
RM4-5++
H129.19++
RM4-4-
Product options: 4
130-109-495

CD4-PE-Vio770, mouse

9 µg in 300 µL (1)
EUR 67,00 
130-109-416

CD4-PE-Vio770, mouse

30 µg in 1 mL (1)
EUR 100,00 
130-109-491

CD4-Biotin, mouse

9 µg in 300 µL (1)
EUR 20,00 
130-109-412

CD4-Biotin, mouse

30 µg in 1 mL (1)
EUR 35,00