CD3ε antibodies – functional grade, mouse

CD3ε antibodies – functional grade, mouse

Clone: 145-2C11
Mouse CD3ε, which is involved in the transduction of activation signals, is expressed on thymocytes, mature T lymphocytes, and NKT cells. CD3ε is a 25 kDa transmembrane protein and a subunit of the CD3 complex. Along with the γ and δ subunits of CD3, the ε chain is required for proper assembly and expression of the T cell receptor complex. The 145-2C11 antibody, which recognizes specifically CD3ε, can activate T cell function.

Applications

The CD3ε functional grade antibodies are suited for functional assays, for example,
in vitro
activation and expansion of T cells in combination with CD2 and CD28 pure – functional grade antibodies, or CD2- and CD28-Biotin functional grade antibodies, and Anti-Biotin MACSiBead™ Particles, cell culture grade. The CD3ε pure – functional grade antibodies, mouse, can be used for
in vivo
T cell depletion.

Alternative Names

CD3e, CD3epsilon, T3E, T3

Technical specifications

  • Antigen: CD3ε
  • Clone: 145-2C11
  • Isotype: hamster IgG1
  • Alternative names of antigen: CD3e, CD3epsilon, T3E, T3
  • Entrez Gene ID: 12501
  • Molecular mass of antigen [kDa]: 19
  • Distribution of antigen: NK cells, T cells, thymocytes
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: Biotin, pure
  • Selected references

    1. Hsu, H. C. et al. (2007) Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies. J. Immunol. 178(8): 5357-5365
    2. Yang, W. et al. (2004) Perturbed homeostasis of peripheral T cells elicits decreased susceptibility to anti-CD3-induced apoptosis in prediabetic nonobese diabetic mice. J. Immunol. 173(7): 4407-4416
    3. Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. U.S.A. 84(5): 1374-1378
    4. Schneider, E. et al. (1997) Hematopoietic changes induced by a single injection of anti-CD3 monoclonal antibody into normal mice. Stem Cells 15(2): 154-160
Product options: 2
130-093-179

CD3ε-Biotin - functional grade, mouse

100 µg in 1 mL
EUR 156,00 
130-092-973

CD3ε pure - functional grade, mouse

500 µg in 1 mL
EUR 336,00