CD38 antibodies, human

CD38 antibodies, human

Clone: REA671 | Dilution: 1:50
Clone REA671 recognizes the human CD38 antigen, a single-chain type II transmembrane glycoprotein with enzymatic activity. It is present on the majority of hematopoietic cells, prevalent during early differentiation and activation processes. Terminally differentiated B cells (plasma cells) express CD38 brightly. Furthermore, CD38 is constitutively expressed in several tissues, for example, brain, muscle, and kidney. CD38, a disease marker for human leukemias and myelomas, plays a role in the pathogenesis and outcome of human immunodeficiency virus infection and chronic lymphocytic leukemia, and controls insulin release and also the development of diabetes. Furthermore, it catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD
+
to ADP-ribose which is essential for the regulation of intracellular Ca
2+
.
Additional information: Clone REA671 displays negligible binding to Fc receptors.

Alternative names

T10, ADPRC 1

Technical specifications

  • Antigen: CD38
  • Clone: REA671
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: T10, ADPRC 1
  • Entrez Gene ID: 952
  • Molecular mass of antigen [kDa]: 34
  • Distribution of antigen: B cells, basophils, bone marrow, brain, kidney, leukocytes, lymphocytes, monocytes, myeloid cells, NK cells, ovary, pancreatic carcinoma cells, placenta, plasma cells, red blood cells, skeletal muscle, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, PE-Vio770, VioBright FITC

Specificity

In order to compare the epitope specificity of REAfinity Clone REA671 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA671
HB7++
HIT2++
IB6++
REA572++
  • Selected references

    1. Jackson, D. G. and Bell, J. I. (1990) Isolation of a cDNA encoding the human CD38 (T10) molecule, a cell surface glycoprotein with an unusual discontinuous pattern of expression during lymphocyte differentiation. J. Immunol. 144(7): 2811-2815
    2. Sathish, V. et al. (2014) Inflammation, caveolae and CD38-mediated calcium regulation in human airway smooth muscle. Biochim. Biophys. Acta 1843(2): 346-351
    3. Malavasi, F. et al. (2008) Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiol. Rev. 88(3): 841-886
Product options: 6
130-117-829

CD38-PE, human

for 30
tests
(1)
EUR 110,00 
130-117-717

CD38-PE, human

for 100
tests
(1)
EUR 265,00 
130-119-159

CD38-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-118-982

CD38-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-119-677

CD38-VioBright FITC, human

for 30
tests
(1)
EUR 120,00 
130-119-578

CD38-VioBright FITC, human

for 100
tests
(1)
EUR 290,00