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Isolated platelets of whole blood from C57BL/6 mice were fixed and then stained with CD321 (JAM1) antibodies or with the corresponding REA Control antibodies (left peak) as well as with CD61 antibodies for 30 minutes at room temperature. CD61+
thrombocytes were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.