CD32 antibodies, human

CD32 antibodies, human

Clone: REA997 | Dilution: 1:50
Clone REA997 recognizes the human CD32 antigen, also known as FcγRII. CD32 is a 40 kDa low affinity receptor for the Fc fragment of aggregated IgG. Due to alternative splicing three different isoforms of CD32 are expressed on hematopoietic cells: FcRIIA, FcRIIB, and FcRIIC. The cytoplasmic domains of FcRIIA and FcRIIC contain an activatory ITAM motif whereas FcRIIB is an inhibitory receptor containing an ITIM motif. Different isoforms of CD32 are expressed on B cells, monocytes, dendritic cells, granulocytes, and platelets. Clone REA997 recognizes all isoforms of CD32 without blocking the binding of immune complexes.
Additional information: Clone REA997 displays negligible binding to Fc receptors.

Alternative names

FCGR2A, FCGR2B, FCGR2C, FcγRII

Technical specifications

  • Antigen: CD32
  • Clone: REA997
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: FCGR2A, FCGR2B, FCGR2C, FcγRII
  • Entrez Gene ID: 2212
  • Molecular mass of antigen [kDa]: 31
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    , baboon, horse
  • Distribution of antigen: B cells, dendritic cells, endothelial cells, granulocytes, leukocytes, macrophages, monocytes, platelets
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, Biotin
  • Selected references

    1. Stuart, S. G. et al. (1989) Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium. EMBO J. 8(12): 3657-3666
    2. Van Den Herik-Oudijk, I. E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes. J. Immunol. 152(2): 574-585
    3. Båve, U. et al. (2003) FcγRIIa is expressed on natural IFN-α-producing cells (plasmacytoid dendritic cells) and is required for the IFN-α production induced by apoptotic cells combined with lupus IgG. J. Immunol. 171: 3296-3302

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