CD314 (NKG2D) antibodies, rat

CD314 (NKG2D) antibodies, rat

Clone: REA471 | Dilution: 1:10
Clone REA471 recognizes the rat CD314 antigen, a single-pass type II membrane homodimeric glycoprotein belonging to the C-type lectin-like receptor family, which is also known as NKG2-D type II integral membrane protein (NKG2D) or killer cell lectin-like receptor subfamily K member 1 (Klrk1). Rat CD314 is expressed in NK cells, and is strongly expressed in resting CD8
+
and CD4
+
T cells, but not by thymocytes or other hemopoietic cells. It functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. CD314 provides both, stimulatory and costimulatory innate immune responses on activated killer cells, leading to cytotoxic activity. It acts as a costimulatory receptor for T cell–receptor in CD8
+
T cell–mediated adaptive immune responses by amplifying T cell–activation. Ligands of CD314 belong to various subfamilies of MHC class I–related glycoproteins.
Additional information: Clone REA471 displays negligible binding to Fc receptors.

Alternative Names

Klrk1, Nkg2d, NKLLR, NKR-P2, NKG2D

Technical specifications

  • Antigen: CD314
  • Clone: REA471
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Klrk1, Nkg2d, NKLLR, NKR-P2, NKG2D
  • Entrez Gene ID: 24934
  • Molecular mass of antigen [kDa]: 24
  • Distribution of antigen: NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, Biotin
  • Selected References

    1. Berg, S. F. et al. (1998) Molecular characterization of rat NKR-P2, a lectin-like receptor expressed by NK cells and resting T cells. Int. Immunol. 10(4): 379-385
    2. Lai, C. B. et al. (2009) Creation of the two isoforms of rodent NKG2D was driven by a B1 retrotransposon insertion. Nucleic Acids Res. 37(9): 3032-3043
    3. Flornes, L. M. et al. (2010) The complete inventory of receptors encoded by the rat natural killer cell gene complex. Immunogenetics 62(8): 521-530
Product options: 8

Product information

Size
Order no.
Price

CD314 (NKG2D)-VioBright FITC, rat

  • REA471
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-993
EUR 56,00 

CD314 (NKG2D)-VioBright FITC, rat

  • REA471
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-106-929
EUR 84,00 

CD314 (NKG2D)-PE, rat

  • REA471
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-991
EUR 40,00 

CD314 (NKG2D)-PE, rat

  • REA471
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-106-927
EUR 58,00 

CD314 (NKG2D)-APC, rat

  • REA471
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-992
EUR 56,00 

CD314 (NKG2D)-APC, rat

  • REA471
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-106-928
EUR 84,00 

CD314 (NKG2D)-Biotin, rat

  • REA471
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-990
EUR 20,00 

CD314 (NKG2D)-Biotin, rat

  • REA471
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-106-926
EUR 32,00