CD303 (BDCA-2) antibodies, human

CD303 (BDCA-2) antibodies, human

Clone: REA693 | Dilution: 1:50
CD303 (BDCA-2) antibodies have been used, for example, to identify, characterize, and enumerate plasmacytoid dendritic cells in whole blood of healthy and HIV-infected individuals, and for analyzing the role of DC-SIGN in HIV infection and transmission. Furthermore, CD303 (BDCA-2) antibodies have been used to identify and enumerate plasmacytoid dendritic cells in blood and bone marrow samples before and after hematopoietic stem cell mobilization or transplantation. CD303 (BDCA-2) antibodies have also been used for immunohistochemical staining, for example, to identify plasmacytoid dendritic cells in tissue sections from patients with different inflammatory skin diseases.
Clone REA693 recognizes the CD303 (BDCA-2) antigen which is expressed on human plasmacytoid dendritic cells in blood, lymphoid (e.g. tonsils and bone marrow), and non-lymphoid tissue. Specific expression allows direct identification of plasmacytoid dendritic cells using just one marker. CD303 (BDCA-2)
+
plasmacytoid dendritic cells in blood and bone marrow are CD11c
, CD123
high
, CD4
+
, Lin
, CD45RA
+
, CD304 (BDCA-4/Neuropilin-1)
+
, CD141 (BDCA-3)
low
, CD1c (BDCA-1)
, CD14
, and CD2
. They express neither myeloid lineage markers (CD13, CD33) nor Fc receptors (CD32, CD64, FcRI). CD303 (BDCA-2) is strongly expressed on freshly isolated plasmacytoid dendritic cells but down-regulated within 48 hours of culturing. Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable on
ex vivo
generated monocyte-derived or hematopoietic precursor cell-derived CD1a
+
dendritic cells.
The CD303 (BDCA-2) antigen is a novel type II transmembrane C-type lectin. Remarkably, plasmacytoid dendritic cells can take up ligands via CD303 (BDCA-2), then process and present the ligands to T cells. Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits type I IFN production, which is induced in plasmacytoid dendritic cells by, for example, the influenza virus.
Additional information: Clone REA693 displays negligible binding to Fc receptors.

Alternative Names

BDCA-2

Technical specifications

  • Antigen: CD303 (BDCA-2)
  • Clone: REA693
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: BDCA-2
  • Entrez Gene ID: 170482
  • Molecular mass of antigen [kDa]: 25
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin
  • Selected References

    1. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J. Immunol. 185(6): 3313-3325
    2. Clark, F. J. et al. (2003) Origin and subset distribution of peripheral blood dendritic cells in patients with chronic graft-versus-host disease. Transplantation 75: 221-225
    3. Krupna-Gaylord, M. A. et al. (2014) Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36. PLoS One 9(6): e100174
    4. Wollenberg, A. et al. (2002) Plasmacytoid dendritic cells: A new cutaneous dendritic cell subset with distinct role in inflammatory skin diseases. J. Invest. Dermatol. 119: 1096-1102
    5. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    6. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63: 1133-1148
    7. Arpinati, M. et al. (2002) Use of anti-BDCA-2 antibody for detection of dendritic cell type-2 (DC2) in allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant. 29: 887-891
    8. Zhang, Y. et al. (2013) The influence of cathelicidin LL37 in human anti-neutrophils cytoplasmic antibody (ANCA)-associated vasculitis. Arthritis Res. Ther. 15(5): R161
    9. Ebner, S. et al. (2004) Expression of C-type lectin receptors by subsets of dendritic cells in human skin. Int. Immunol. 16: 877-887
    10. Nestle, F.O. et al. (2005) Plasmacytoid predendritic cells initiate psoriasis through interferon-α production. J. Exp. Med. 202: 135-143
    11. Rönnblom, L. and Alm, G. V. (2001) A pivotal role for the natural interferon α-producing cells (plasmacytoid dendritic cells) in the pathogenesis of lupus. J. Exp. Med. 194: 59-63
    12. Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823-1834
    13. De Wit, D. et al. (2004) Blood plasmacytoid dendritic cell responses to CpG oligonucleotides are impaired in human newbornes. Blood 103: 1030-1032
    14. Dutertre, C. A. et al. (2012)
      Pivotal role of M-DC8
      +
      monocytes from viremic HIV-infected patients in TNFα overproduction in response to microbial products.
      Blood 120(11): 2259-2268
    15. Chehimi, J. et al. (2002) Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individuals. J. Immunol. 168: 4769-4801
    16. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    17. Ebner, S. et al. (2002) A novel role for IL-13: Human monocytes cultured in the presence of IL-3 and IL-4 differentiate into dendritic cells that produce less IL-12 and shift the cell responses toward a Tʜ2 cytokine pattern. J. Immunol. 168: 6199-6207
    18. Vanders, R. L. et al. (2013) Plasmacytoid dendritic cells and CD8 T cells from pregnant women show altered phenotype and function following H1N1/09 infection. J. Infect. Dis. 208(7): 1062-1070
Product options: 12

Product information

Size
Order no.
Price

CD303 (BDCA-2)-VioBright FITC, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-182
EUR 240,00 

CD303 (BDCA-2)-VioBright FITC, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-656
EUR 450,00 

CD303 (BDCA-2)-PE, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-180
EUR 160,00 

CD303 (BDCA-2)-PE, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-654
EUR 424,00 

CD303 (BDCA-2)-APC, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-177
EUR 211,00 

CD303 (BDCA-2)-APC, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-651
EUR 444,00 

CD303 (BDCA-2)-PE-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-181
EUR 211,00 

CD303 (BDCA-2)-PE-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-655
EUR 444,00 

CD303 (BDCA-2)-APC-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-178
EUR 206,00 

CD303 (BDCA-2)-APC-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-652
EUR 430,00 

CD303 (BDCA-2)-Biotin, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-179
EUR 125,00 

CD303 (BDCA-2)-Biotin, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-653
EUR 342,00