CD303 (BDCA-2) antibodies, human

CD303 (BDCA-2) antibodies, human

Clone: AC144 | Dilution: 1:11
CD303 (BDCA-2) antibodies have been used, for example, to identify, characterize, and enumerate plasmacytoid dendritic cells in whole blood of healthy and HIV-infected individuals, and for analyzing the role of DC-SIGN in HIV infection and transmission.
7,11
Furthermore, CD303 (BDCA-2) antibodies were used to identify and enumerate plasmacytoid dendritic cells in blood and bone marrow samples before and after hematopoietic stem cell mobilization
8
or transplantation
10
. CD303 (BDCA-2) antibodies were also used for immunohistochemical staining, for example to identify plasmacytoid dendritic cells in tissue sections from patients with different inflammatory skin diseases.
5,12,13
Clone AC144 recognizes the the CD303 (BDCA-2) antigen
1,3
which is expressed on human plasmacytoid dendritic cells in blood, lymphoid (e.g. tonsils and bone marrow) and non-lymphoid tissue.
3,6
Specific expression allows direct identification of plasmacytoid dendritic cells using just one marker.
1–11
CD303 (BDCA-2)
+
plasmacytoid dendritic cells in blood and bone marrow are CD11c
, CD123
high
, CD4
+
, Lin
, CD45RA
+
, CD304 (BDCA-4/Neuropilin-1)
+
, CD141 (BDCA-3)
low
, CD1c (BDCA-1)
, CD14
, and CD2
. They express neither myeloid lineage markers (CD13, CD33) nor Fc receptors (CD32, CD64, FcRI).
1,6
CD303 (BDCA-2) is strongly expressed on freshly isolated plasmacytoid dendritic cells but down-regulated within 48 hours of culturing.
1
Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable on
ex vivo
generated monocyte-derived or hematopoietic precursor cell-derived CD1a
+
dendritic cells.
1,9
The CD303 (BDCA-2) antigen is a novel type II transmembrane C-type lectin.
3
Remarkably, plasmacytoid dendritic cells can take up ligands via CD303 (BDCA-2), then process and present the ligands to T cells.
1,3
Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits type I IFN production, which is induced in plasmacytoid dendritic cells by, for example, the influenza virus.
3,6

Alternative names

CLEC4C, BDCA-2, CLECSF11, CLECSF7, DLEC, HECL, PRO34150

Technical specifications

  • Antigen: CD303 (BDCA-2)
  • Clone: AC144
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: CLEC4C, BDCA-2, CLECSF11, CLECSF7, DLEC, HECL, PRO34150
  • Entrez Gene ID: 170482
  • Molecular mass of antigen [kDa]: 25
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, VioBlue, pure
  • Selected references

    1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    2. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    3. Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823-1834
    4. Rönnblom, L. and Alm, G. V. (2001) A pivotal role for the natural interferon α-producing cells (plasmacytoid dendritic cells) in the pathogenesis of lupus. J. Exp. Med. 194: 59-63
    5. Wollenberg, A. et al. (2002) Plasmacytoid dendritic cells: A new cutaneous dendritic cell subset with distinct role in inflammatory skin diseases. J. Invest. Dermatol. 119: 1096-1102
    6. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 36: 1133-1148
    7. Chehimi, J. et al. (2002) Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individuals. J. Immunol. 168: 4769-4801
    8. Arpinati, M. et al. (2002) Use of anti-BDCA-2 antibody for detection of dendritic cell type-2 (DC2) in allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant. 29: 887-891
    9. Ebner, S. et al. (2002) A novel role for IL-13: Human monocytes cultured in the presence of IL-3 and IL-4 differentiate into dendritic cells that produce less IL-12 and shift the cell responses toward a Tʜ2 cytokine pattern. J. Immunol. 168: 6199-6207
    10. Clark, F. J. et al. (2003) Origin and subset distribution of peripheral blood dendritic cells in patients with chronic graft-versus-host disease. Transplantation 75: 221-225
    11. De Wit, D. et al. (2004) Blood plasmacytoid dendritic cell responses to CpG oligonucleotides are impaired in human newbornes. Blood 103: 1030-1032
    12. Nestle, F.O. et al. (2005) Plasmacytoid predendritic cells initiate psoriasis through interferon-α production. J. Exp. Med. 202: 135-143
    13. Ebner, S. et al. (2004) Expression of C-type lectin receptors by subsets of dendritic cells in human skin. Int. Immunol. 16: 877-887
    14. Vanders, R. L. et al. (2013) Plasmacytoid dendritic cells and CD8 T cells from pregnant women show altered phenotype and function following H1N1/09 infection. J. Infect. Dis. 208(7): 1062-1070
    15. Dutertre, C. A. et al. (2012)
      Pivotal role of M-DC8
      +
      monocytes from viremic HIV-infected patients in TNFα overproduction in response to microbial products.
      Blood 120(11): 2259-2268
    16. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J. Immunol. 185(6): 3313-3325
    17. Zhang, Y. et al. (2013) The influence of cathelicidin LL37 in human anti-neutrophils cytoplasmic antibody (ANCA)-associated vasculitis. Arthritis Res. Ther. 15(5): R161
    18. Krupna-Gaylord, M. A. et al. (2014) Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36. PLoS One 9(6): e100174
    19. Kuzin, I. et al. (2011) Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor. Biotechnol. Bioeng. 108(6): 1430-1440
  • Customer reports and application notes

Product options: 4
130-097-929

CD303 (BDCA-2)-PE, human

for 30
tests
(1)
EUR 133,00 
130-106-505

CD303 (BDCA-2)-VioBlue, human

for 30
tests
(1)
EUR 152,00 
130-097-323

CD303 (BDCA-2)-VioBlue, human

for 100
tests
(1)
EUR 400,00 
130-090-690

CD303 (BDCA-2) pure, human

100 µg in 1 mL
EUR 137,00