CD284 antibodies, human

CD284 antibodies, human

Clone: HTA125 | Dilution: 1:11
Clone HTA125 reacts with CD284, a type I transmembrane signaling receptor also known as Toll-like receptor 4 (TLR4). Toll-like receptors are a family of pattern recognition receptors detecting the presence and characteristics of an infection and induce appropriately tailored innate and adaptive immune responses. TLR4 associates with its co-receptor MD-2. Together with the lipopolysaccharide (LPS)-binding protein (LBP) and CD14, this complex is responsible for signaling upon LPS recognition. TLR4 is expressed on monocytes and myeloid dendritic cells. Low levels are expressed on B cells and granulocytes in human peripheral blood. HTA125 is able to block LPS-induced signaling and recognizes predominantly human TLR4 associated with MD-2.

Alternative names

TLR4, ARMD10, TLR-4, TOLL

Technical specifications

  • Antigen: CD284
  • Clone: HTA125
  • Isotype: mouse IgG2aκ
  • Isotype control: Mouse IgG2a – isotype control antibodies
  • Alternative names of antigen: TLR4, ARMD10, TLR-4, TOLL
  • Entrez Gene ID: 7099
  • Molecular mass of antigen [kDa]: 93
  • Cross-reactivity: rabbit, guinea pig
  • Distribution of antigen: dendritic cells, endothelial cells, granulocytes, leukocytes, macrophages, monocytes, placenta, spleen, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, Biotin
  • Selected references

    1. Shimazu, R. et al. (1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J. Exp. Med. 189: 1777-1782
    2. Kawahara, T. et al. (2001)
      Type I
      Helicobacter pylori
      lipopolysaccharide stimulates Toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells.
      Infect. Immun. 69: 4382-4389
    3. Wang, J. et al. (2001)
      Involvement of CD14 and Toll-Like receptors in activation of human monocytes by
      Aspergillus fumigatus
      hyphae.
      Infect. Immun. 69: 2402-2406
    4. Wang, R. et al. (2003) Characterization of monoclonal antibody HTA125 with specificity for human TLR4. Hybrid. Hybridomics 22: 357-365
    5. Jin, M. S. and Lee, J-O. (2008) Structures of the toll-like receptor family and its ligand complexes. Immunity 29: 182-191
    6. Burgener, I. et al. (2008) Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet. Immunol. Immunopathol. 124: 184-191
Product options: 5
130-100-378

CD284-PE, human

for 30
tests
(1)
EUR 110,00 
130-096-237

CD284-PE, human

for 100
tests
(1)
EUR 265,00 
130-100-150

CD284-APC, human

for 30
tests
(1)
EUR 110,00 
130-096-236

CD284-APC, human

for 100
tests
(1)
EUR 265,00 
130-097-349

CD284-Biotin, human

for 100
tests
(1)
EUR 220,00