CD28 antibodies – functional grade, human

CD28 antibodies – functional grade, human

Clone: 15E8
The CD28 antibody recognizes the human CD28 antigen, which is highly expressed on CD3
+
thymocytes, most peripheral thymocytes, and plasma cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production
in vitro
and
in vivo
.
In the presence of antibodies directed against CD2 and CD3, CD28 antibodies stimulate T cell proliferation and cytokine production.
Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of antigen-presenting cells (APCs). The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency.

Applications

The CD28 antibodies – functional grade are suited for functional assays, for example,
in vitro
activation and expansion of T cells in combination with CD2 and CD3 pure – functional grade antibodies, or CD2- and CD3-Biotin – functional grade antibodies, and Anti-Biotin MACSiBead™ Particles, cell culture grade.

Alternative names

Tp44, T44

Technical specifications

  • Antigen: CD28
  • Clone: 15E8
  • Isotype: mouse IgG1
  • Alternative names of antigen: Tp44, T44
  • Entrez Gene ID: 940
  • Molecular mass of antigen [kDa]: 23
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: B cells, lymphocytes, plasma cells, T cells, thymocytes
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Kozbor D. J. et al. (1987) Tp44 molecules involved in antigen-independent T cell activation are expressed on human plasma cells. J. Immunol. 138(12): 4128-4132
    2. Bahlis, N. J. et al. (2007) CD28-mediated regulation of multiple myeloma cell proliferation and survival. Blood 109(11): 5002-5010
    3. Lécureuil et al. (2007) Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood 109: 3649-3657
    4. Krummel, M. F. and Allison, J. P. (1995) CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation. J. Exp. Med. 182(2): 459-465
    5. Freeman, G. J. et al. (1993) Cloning of B7-2: a CTLA-4 counter-receptor that costimulates human T cell proliferation. Science 262(5135): 909-911
    6. Linsley, P. S. et al. (1993) The role of the CD28 receptor during T cell responses to antigen. Annu. Rev. Immunol. 11: 191-212
    7. June, C. H. et al. (1990) Role of the CD28 receptor in T-cell activation. Immunol. Today 11(6): 211-216
  • Customer reports and application notes

Product options: 2
130-093-386

CD28-Biotin - functional grade, human

100 µg in 1 mL
EUR 256,00 
130-093-375

CD28 pure - functional grade, human

100 µg in 1 mL
EUR 165,00