CD28 antibodies – functional grade, human

CD28 antibodies – functional grade, human

Clone: 15E8
The CD28 antibody recognizes the human CD28 antigen, which is highly expressed on CD3
thymocytes, most peripheral thymocytes, and plasma cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production
in vitro
in vivo
In the presence of antibodies directed against CD2 and CD3, CD28 antibodies stimulate T cell proliferation and cytokine production.
Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of antigen-presenting cells (APCs). The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency.


The CD28 antibodies – functional grade are suited for functional assays, for example,
in vitro
activation and expansion of T cells in combination with CD2 and CD3 pure – functional grade antibodies, or CD2- and CD3-Biotin – functional grade antibodies, and Anti-Biotin MACSiBead™ Particles, cell culture grade.

Alternative names

Tp44, T44

Technical specifications

  • Antigen: CD28
  • Clone: 15E8
  • Isotype: mouse IgG1
  • Alternative names of antigen: Tp44, T44
  • Entrez Gene ID: 940
  • Molecular mass of antigen [kDa]: 23
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    cynomolgus monkey (
    Macaca fascicularis
  • Distribution of antigen: B cells, lymphocytes, plasma cells, T cells, thymocytes
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Kozbor D. J. et al. (1987) Tp44 molecules involved in antigen-independent T cell activation are expressed on human plasma cells. J. Immunol. 138(12): 4128-4132
    2. Bahlis, N. J. et al. (2007) CD28-mediated regulation of multiple myeloma cell proliferation and survival. Blood 109(11): 5002-5010
    3. Lécureuil et al. (2007) Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood 109: 3649-3657
    4. Krummel, M. F. and Allison, J. P. (1995) CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation. J. Exp. Med. 182(2): 459-465
    5. Freeman, G. J. et al. (1993) Cloning of B7-2: a CTLA-4 counter-receptor that costimulates human T cell proliferation. Science 262(5135): 909-911
    6. Linsley, P. S. et al. (1993) The role of the CD28 receptor during T cell responses to antigen. Annu. Rev. Immunol. 11: 191-212
    7. June, C. H. et al. (1990) Role of the CD28 receptor in T-cell activation. Immunol. Today 11(6): 211-216
  • Customer reports and application notes

Product options: 2

CD28-Biotin - functional grade, human

100 µg in 1 mL
EUR 256,00 

CD28 pure - functional grade, human

100 µg in 1 mL
EUR 165,00