CD278 (ICOS) antibodies, human, mouse, rat

CD278 (ICOS) antibodies, human, mouse, rat

Clone: REA192 | Dilution: 1:11
Clone REA192 recognizes CD278 (ICOS), which belongs to the CD28 family of proteins. CD28 family members are co-stimulatory receptor molecules, contain single immunoglobulin V-like domain, and belong to the immunoglobulin superfamily. CD287 recognizes ICOSL (inducible T cell co-stimulator ligand) as ligand and its expression is induced on activated T cells. Enhanced expression of CD278 is also observed on T cells, followed by co-stimulation of T cells via CD28. Further expression of CD278 is found on B cells, monocytes, and dendritic cells. Crosslinking of ICOS on T cell surface in the presence of primary stimulation signal leads to T cell proliferation and differentiation. In addition, CD278 plays role in Tʜ cell differentiation, effector T cell function, and isotype class switching.
Additional information: Clone REA192 displays negligible binding to Fc receptors.

Alternative Names

ICOS, AILIM, CVID1

Technical specifications

  • Antigen: CD278 (ICOS)
  • Clone: REA192
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: ICOS, AILIM, CVID1
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
  • Selected References

    1. Akbari, O. et al. (2008)
      ICOS/ICOSL interaction is required for CD4
      +
      invariant NKT cell function and homeostatic survival
      J. Immunol. 180: 5448-5456
    2. Wassink, L. et al. (2004) ICOS expression by activated human Tʜ Cells is enhanced by IL-12 and IL-23: Increased ICOS expression enhances the effector function of both Tʜ1 and Tʜ2 cells. J. Immunol. 173: 1779-1786

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA192
DX29++
ISA-3++
C398.4A++
Product options: 18
130-100-734

CD278 (ICOS)-FITC, human, mouse, rat

for 30
tests
(1)
EUR 90,00 
130-100-732

CD278 (ICOS)-FITC, human, mouse, rat

for 100
tests
(1)
EUR 220,00 
130-100-719

CD278 (ICOS)-APC, human, mouse, rat

for 30
tests
(1)
EUR 110,00 
130-100-721

CD278 (ICOS)-APC, human, mouse, rat

for 100
tests
(1)
EUR 265,00 
130-100-639

CD278 (ICOS)-VioBlue, human, mouse, rat

for 30
tests
(1)
EUR 115,00 
130-100-737

CD278 (ICOS)-VioBlue, human, mouse, rat

for 100
tests
(1)
EUR 275,00 
130-100-740

CD278 (ICOS)-VioGreen, human, mouse, rat

for 30
tests
(1)
EUR 115,00 
130-100-739

CD278 (ICOS)-VioGreen, human, mouse, rat

for 100
tests
(1)
EUR 275,00 
130-107-512

CD278 (ICOS)-PE-Vio615, human, mouse, rat

for 30
tests
(1)
EUR 120,00 
130-107-458

CD278 (ICOS)-PE-Vio615, human, mouse, rat

for 100
tests
(1)
EUR 290,00 
130-100-736

CD278 (ICOS)-PE-Vio770, human, mouse, rat

for 30
tests
(1)
EUR 120,00 
130-100-735

CD278 (ICOS)-PE-Vio770, human, mouse, rat

for 100
tests
(1)
EUR 290,00 
130-100-727

CD278 (ICOS)-APC-Vio770, human, mouse, rat

for 30
tests
(1)
EUR 120,00 
130-100-723

CD278 (ICOS)-APC-Vio770, human, mouse, rat

for 100
tests
(1)
EUR 290,00 
130-100-733

CD278 (ICOS)-PerCP-Vio700, human, mouse, rat

for 30
tests
(1)
EUR 120,00 
130-100-731

CD278 (ICOS)-PerCP-Vio700, human, mouse, rat

for 100
tests
(1)
EUR 290,00 
130-100-726

CD278 (ICOS)-Biotin, human, mouse, rat

for 30
tests
(1)
EUR 90,00 
130-100-728

CD278 (ICOS)-Biotin, human, mouse, rat

for 100
tests
(1)
EUR 220,00