Name: CD25 antibodies, mouse
Availability: InStock

Product overview

Clone REA568 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4

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CD25

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regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs).

Additional information: Clone REA568 displays negligible binding to Fc receptors.

Alternative names

IL2RA, IL2R, Ly-43, p55

Detailed product information

Technical specifications

Antigen: CD25
Clone: REA568
Isotype: recombinant human IgG1
Isotype control: REA Control antibodies
Alternative names of antigen: IL2RA, IL2R, Ly-43, p55
Entrez Gene ID: 16184
Molecular mass of antigen [kDa]: 28
Distribution of antigen: T cells, B cells, dendritic cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: VioBright 667, VioBright FITC, PE, APC, PE-Vio615, PE-Vio770, Biotin

Product data and images

Figure 1

Splenocytes from C57BL/6 mice were stained with CD25 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Scientific resources

Selected references
1. Miller, J. et al. (1985) Nucleotide sequence and expression of a mouse interleukin 2 receptor cDNA. J. Immunol. 134(6): 4212-4217
2. Read, S. et al. (2000)
  Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25
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  CD4
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  regulatory cells that control intestinal inflammation.
  J. Exp. Med. 192(2): 295-302
3. Taniguchi, T. et al. (1993) The IL-2/IL-2 receptor system: a current overview. Cell 73(1): 5-8

Validation data

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones.