CD25 antibodies, mouse

CD25 antibodies, mouse

Clone: REA568 | Dilution: 1:50
Clone REA568 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4
+
CD25
+
regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs).
Additional information: Clone REA568 displays negligible binding to Fc receptors.

Alternative Names

IL2RA, IL2R, Ly-43, p55

Technical specifications

  • Antigen: CD25
  • Clone: REA568
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: IL2RA, IL2R, Ly-43, p55
  • Entrez Gene ID: 16184
  • Molecular mass of antigen [kDa]: 28
  • Distribution of antigen: T cells, B cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright 667, VioBright FITC, PE, APC

  • Figure 1

    Splenocytes from C57BL/6 mice were stained with CD25 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant
    ®
    Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clonesOverlap in epitope recognition with REA568
3C7+
7D4++
PC61-

Related products

2 products available
Product options: 8

Product information

Size
Order no.
Price

CD25-VioBright 667, mouse

  • REA568
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-118-376
EUR 108,00 

CD25-VioBright 667, mouse

  • REA568
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-118-222
EUR 396,00 

CD25-VioBright FITC, mouse

  • REA568
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-120-172
EUR 90,00 

CD25-VioBright FITC, mouse

  • REA568
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-120-088
EUR 265,00 

CD25-PE, mouse

  • REA568
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-120-766
EUR 75,00 

CD25-PE, mouse

  • REA568
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-120-696
EUR 220,00 

CD25-APC, mouse

  • REA568
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-120-767
EUR 75,00 

CD25-APC, mouse

  • REA568
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-120-697
EUR 220,00 
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