CD25 antibodies, mouse

CD25 antibodies, mouse

Clone: REA568 | Dilution: 1:50
Clone REA568 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4
+
CD25
+
regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs).
Additional information: Clone REA568 displays negligible binding to Fc receptors.

Alternative names

IL2RA, IL2R, Ly-43, p55

Technical specifications

  • Antigen: CD25
  • Clone: REA568
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: IL2RA, IL2R, Ly-43, p55
  • Entrez Gene ID: 16184
  • Molecular mass of antigen [kDa]: 28
  • Distribution of antigen: T cells, B cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright 667, VioBright FITC, PE, APC, PE-Vio770, Biotin

  • Figure 1

    Splenocytes from C57BL/6 mice were stained with CD25 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant
    ®
    Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  • Selected references

    1. Miller, J. et al. (1985) Nucleotide sequence and expression of a mouse interleukin 2 receptor cDNA. J. Immunol. 134(6): 4212-4217
    2. Read, S. et al. (2000)
      Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25
      +
      CD4
      +
      regulatory cells that control intestinal inflammation.
      J. Exp. Med. 192(2): 295-302
    3. Taniguchi, T. et al. (1993) The IL-2/IL-2 receptor system: a current overview. Cell 73(1): 5-8
Product options: 10
130-118-376

CD25-VioBright 667, mouse

30 µg in 200 µL (1)
EUR 145,00 
130-118-222

CD25-VioBright 667, mouse

150 µg in 1 mL (1)
EUR 420,00 
130-120-172

CD25-VioBright FITC, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-120-088

CD25-VioBright FITC, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-120-766

CD25-PE, mouse

30 µg in 200 µL (1)
EUR 80,00 
130-120-696

CD25-PE, mouse

150 µg in 1 mL (1)
EUR 230,00 
130-120-767

CD25-APC, mouse

30 µg in 200 µL (1)
EUR 80,00 
130-120-697

CD25-APC, mouse

150 µg in 1 mL (1)
EUR 230,00 
130-123-893

CD25-PE-Vio770, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-123-860

CD25-Biotin, mouse

30 µg in 200 µL (1)
EUR 40,00 

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