CD25 antibodies, mouse

Overview

Clone REA568 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4

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CD25

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regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs).

Additional information: Clone REA568 displays negligible binding to Fc receptors.

Alternative Names

IL2RA, IL2R, Ly-43, p55

Details

Technical specifications

Antigen: CD25
Clone: REA568
Isotype: recombinant human IgG1
Isotype control: REA Control antibodies
Alternative names of antigen: IL2RA, IL2R, Ly-43, p55
Entrez Gene ID: 16184
Molecular mass of antigen [kDa]: 28
Distribution of antigen: T cells, B cells, dendritic cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: VioBright 667

Gallery

Figure 1

Splenocytes from C57BL/6 mice were stained with CD25 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.