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Miltenyi Biotec Inc.
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Phone: +1 800 FOR MACS
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E-Mail: science@miltenyibiotec.com
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Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Splenocytes from C57BL/6 mice were stained with CD25 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones.
| Other clones | Overlap in epitope recognition with REA568 |
| 3C7 | + |
| 7D4 | ++ |
| PC61 | - |
