CD25 antibodies, human

CD25 antibodies, human

Clone: REA945 | Dilution: 1:50
Clone REA945 recognizes the human CD25 antigen, a 28 kDa glycoprotein also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4
+
regulatory T cells. CD25 plays an essential role in the regulation of immune tolerance by controlling regulatory T cells activity. The CD25 antigen contains three epitope regions called A, B, and C. Clone REA945 recognizes epitope region B.
Additional information: Clone REA945 displays negligible binding to Fc receptors.

Alternative names

IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα

Technical specifications

  • Antigen: CD25
  • Clone: REA945
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα
  • Entrez Gene ID: 3559
  • Molecular mass of antigen [kDa]: 28
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
  • Distribution of antigen: myeloid cells, B cells, granulocytes, lymphocytes, macrophages, monocytes, NK cells, osteoclasts, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio615, PE-Vio770, VioBright 515, Biotin
  • Selected references

    1. Sakaguchi, S. et al. (1995) Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor α-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J. Immunol. 155: 1151-1164
    2. Shevach, E. M. (2001)
      Certified professionals: CD4
      +
      CD25
      +
      suppressor T cells.
      J. Exp. Med. 193(11): F41-F46
    3. Maloy, K. J. et al. (2001) Regulatory T cells in the control of immune pathology. Nat. Immunol. 2(9): 816-822
    4. Lamprecht, B. et al. (2008) Aberrant expression of the Tʜ2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha. Blood 112(8): 3339-3347
    5. Chaput, N. et al. (2013)
      Phase I clinical trial combining imatinib mesylate and IL-2: HLA-DR
      +
      NK cell levels correlate with disease outcome.
      Oncoimmunology 2(2): e23080
Product options: 11
130-115-628

CD25-PE, human

for 30
tests
(1)
EUR 95,00 
130-115-534

CD25-PE, human

for 100
tests
(1)
EUR 235,00 
130-115-535

CD25-APC, human

for 100
tests
(1)
EUR 235,00 
for 30
tests
(1)
EUR 120,00 
for 100
tests
(1)
EUR 290,00 
for 30
tests
(1)
EUR 120,00 
for 100
tests
(1)
EUR 290,00 
for 30
tests
(1)
EUR 150,00 
for 100
tests
(1)
EUR 360,00 
for 30
tests
(1)
EUR 70,00 
for 100
tests
(1)
EUR 175,00