CD25 antibodies, human

CD25 antibodies, human

Clone: REA570 | Dilution: 1:11
Clone REA570 recognizes the human CD25 glycoprotein, also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). It associates with IL-2 receptor β (CD122) and γ chains (CD132) to form a high affinity IL-2R complex. CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4
+
regulatory T cells. Clone REA570 recognizes epitope region A.
Additional information: Clone REA570 displays negligible binding to Fc receptors

Alternative Names

IL2RA, IDDM10, IL2R, TCGFR, p55, Tac

Technical specifications

  • Antigen: CD25
  • Clone: REA570
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL2RA, IDDM10, IL2R, TCGFR, p55, Tac
  • Entrez Gene ID: 3559
  • Molecular mass of antigen [kDa]: 55
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    olive baboon (
    Papio anubis
    )
  • Distribution of antigen: T cells, B cells, macrophages
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, VioBright 667

Specificity

In order to compare the epitope specificity of REAfinity Clone REA570 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA570
2A3++
M-A251-
4E3-
BC96++
3G10++
REA945-
  • Selected references

    1. Piccirillo, C. A. et al. (2006)
      Development and function of naturally occurring CD4
      +
      CD25
      +
      regulatory T cells.
      J. Leukoc. Biol. 3: 458-470
    2. Lee, S. H. et al. (2012) Cutting edge: a novel mechanism bridging innate and adaptive immunity: IL-12 induction of CD25 to form high-affinity IL-2 receptors on NK cells. J. Immunol. 189(6): 2712-2716
    3. Goudy, K. et al. (2013) Human IL2RA null mutation mediates immunodeficiency with lymphoproliferation and autoimmunity. Clin. Immunol. 146(3): 248-261
Product options: 2
130-111-925

CD25-VioBright 667, human

for 30
tests
(1)
EUR 150,00 
130-111-754

CD25-VioBright 667, human

for 100
tests
(1)
EUR 360,00 

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