CD203c antibodies, human

CD203c antibodies, human

Clone: REA826 | Dilution: 1:50
Clone REA826 recognizes the human CD203c antigen, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils, mast cells, and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g., in flow cytometric basophil activation tests of the peripheral blood.
Additional information: Clone REA826 displays negligible binding to Fc receptors.

Alternative Names

ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b

Technical specifications

  • Antigen: CD203c
  • Clone: REA826
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b
  • Entrez Gene ID: 5169
  • Molecular mass of antigen [kDa]: 100
  • Distribution of antigen: basophils, liver, mast cells, myeloid cells, pancreas, small intestine
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio615, Biotin
  • Selected References

    1. Goding, J. W. (2000) Ecto-enzymes: physiology meets pathology. J. Leukoc. Biol. 67: 285-311
    2. Kahlert, H. et al. (2003) Measurement of basophil-activating capacity of grass pollen allergens, allergoids Clin. Exp. Allergy 33: 1266-1272
    3. Bühring, H. J. et al. (2004) The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int. Arch. Allergy Immunol. 133: 317-329
    4. Hauswirth, A. W. et al. (2002) Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J. Allergy Clin. Immunol. 110: 102-109
Product options: 8

Product information

Size
Order no.
Price

CD203c-PE, human

  • REA826
  • 1:50
  • For research use only
for 30
tests
(1)
130-112-813
EUR 110,00 

CD203c-PE, human

  • REA826
  • 1:50
  • For research use only
for 100
tests
(1)
130-112-624
EUR 265,00 

CD203c-APC, human

  • REA826
  • 1:50
  • For research use only
for 30
tests
(1)
130-112-814
EUR 110,00 

CD203c-APC, human

  • REA826
  • 1:50
  • For research use only
for 100
tests
(1)
130-112-625
EUR 265,00 

CD203c-PE-Vio615, human

  • REA826
  • 1:50
  • For research use only
for 30
tests
(1)
130-112-817
EUR 120,00 

CD203c-PE-Vio615, human

  • REA826
  • 1:50
  • For research use only
for 100
tests
(1)
130-112-628
EUR 290,00 

CD203c-Biotin, human

  • REA826
  • 1:50
  • For research use only
for 30
tests
(1)
130-112-811
EUR 90,00 

CD203c-Biotin, human

  • REA826
  • 1:50
  • For research use only
for 100
tests
(1)
130-112-622
EUR 220,00