CD194 (CCR4) antibodies, human

CD194 (CCR4) antibodies, human

Clone: REA279 | Dilution: 1:50
Clone REA279 recognizes the CD194 antigen, a seven-transmembrane G-protein–coupled receptor also known as C-C chemokine receptor type 4 (CCR4). CD194 is expressed on activated Tʜ2 cells, regulatory T cells, a subset of B cells, activated NK cells, basophils, monocytes, NK cells, and platelets. It is expressed at high levels by skin-infiltrating lymphocytes, at lower levels by lung and synovial fluid lymphocytes, but never by intestinal lymphocytes. CD194 is the specific receptor for the chemokines CCL17 (TARC) and CCL22 (MDC). Human peripheral blood regulatory T cells can be divided into two distinct populations based on the expression of CCR4. The majority of freshly isolated regulatory T cells express CD194 and represent memory-type regulatory T cells, while CD194 regulatory T cells require anti-CD3 antibody–mediated activation to acquire a regulatory activity. Depletion of 194
+
T cells leads to Tʜ1-type polarization of CD4
+
T cells and augmentation of CD8
+
T cell responses to tumor antigens.
Additional information: Clone REA279 displays negligible binding to Fc receptors.

Alternative Names

CCR4, CC-CKR-4, CKR4, CMKBR4, CHEMR13, K5-5

Technical specifications

  • Antigen: CD194 (CCR4)
  • Clone: REA279
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CCR4, CC-CKR-4, CKR4, CMKBR4, CHEMR13, K5-5
  • Entrez Gene ID: 1233
  • Molecular mass of antigen [kDa]: 41
  • Distribution of antigen: T cells, B cells, NK cells, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE-Vio770, PE
  • Selected References

    1. Baatar, D. et al. (2007)
      Human peripheral blood T regulatory cells (Tregs), functionally primed CCR4
      +
      Tregs and unprimed CCR4
      Tregs, regulate effector T cells using FasL.
      J. Immunol. 178(8): 4891-4900
    2. Sallusto, F. et al. (1998) Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes. J. Exp. Med. 187(6): 875-883
    3. Kunkel, E. J. et al. (2002) Expression of the chemokine receptors CCR4, CCR5, and CXCR3 by human tissue-infiltrating lymphocytes. Am. J. Pathol. 160(1): 347-355
    4. Power, C. A. et al. (1995) Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. J. Biol. Chem. 270(33): 19495-19500

Specificity

In order to compare the epitope specificity of REAfinity Clone REA279 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA279
1G1++
L291H4-
Product options: 6
130-117-525

CD194 (CCR4)-APC, human

for 30
tests
(1)
EUR 110,00 
130-117-376

CD194 (CCR4)-APC, human

for 100
tests
(1)
EUR 265,00 
130-118-496

CD194 (CCR4)-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-118-359

CD194 (CCR4)-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-120-596

CD194 (CCR4)-PE, human

for 30
tests
(1)
EUR 110,00 
130-120-456

CD194 (CCR4)-PE, human

for 100
tests
(1)
EUR 265,00