CD185 (CXCR5) antibodies, mouse

CD185 (CXCR5) antibodies, mouse

Clone: REA215 | Dilution: 1:50
Clone REA215 recognizes murine CD185, which is a 42 kDa multipass chemokine receptor, also known as CXCR5 or burkitt lymphoma receptor-1 (BLR). Expression of CD185 is found on mature recirculating B cells, a small subset of CD4
+
and CD8
+
T cells, and skin-derived migratory dendritic cells. CXCR5 recognizes B cell–attracting chemokine 1 (BCA-1)/ CXCL13 as ligand, which is a homeostatic chemokine, constitutively secreted by stromal cells in B cell areas of secondary lymphoid tissues (follicles). CXCR5 is involved in recruitment of circulating naive B cells to follicles and trafficking and homing of B1 B cells. Mice lacking CXCR5 gene display defective primary follicles and germinal centers formation in the spleen and Peyer patches, and lack inguinal lymph nodes.
Additional information: Clone REA215 displays negligible binding to Fc receptors.

Alternative Names

CXCR5, BLR1, CXC-R5, CXCR-5, Gpcr6, MDR15

Technical specifications

  • Antigen: CD185 (CXCR5)
  • Clone: REA215
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CXCR5, BLR1, CXC-R5, CXCR-5, Gpcr6, MDR15
  • Distribution of antigen: B cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE-Vio770, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA215 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA215
L138D7++
2G8++
SPRCL5++
  • Selected References

    1. Junt, T. et al. (2005) CXCR5-dependent seeding of follicular niches by B and Th cells augments antiviral B cell responses. J. Immunol. 175: 7109-7116
    2. Velaga, S. et al. (2009) Chemokine receptor CXCR5 supports solitary intestinal lymphoid tissue formation, B cell homing, and induction of intestinal IgA responses. J. Immunol. 182: 2610-2619
Product options: 5
130-119-129

CD185 (CXCR5)-APC, mouse

30 µg in 200 µL (1)
EUR 80,00 
130-117-515

CD185 (CXCR5)-PE-Vio770, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-117-366

CD185 (CXCR5)-PE-Vio770, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-116-511

CD185 (CXCR5)-Biotin, mouse

30 µg in 200 µL (1)
EUR 40,00 
130-116-540

CD185 (CXCR5)-Biotin, mouse

150 µg in 1 mL (1)
EUR 120,00