CD185 (CXCR5) antibodies, mouse

CD185 (CXCR5) antibodies, mouse

Clone: REA215 | Dilution: 1:10
Clone REA215 recognizes murine CD185, which is a 42 kDa multipass chemokine receptor, also known as CXCR5 or burkitt lymphoma receptor-1 (BLR). Expression of CD185 is found on mature recirculating B cells, a small subset of CD4
+
and CD8
+
T cells, and skin-derived migratory dendritic cells. CXCR5 recognizes B cell–attracting chemokine 1 (BCA-1)/ CXCL13 as ligand, which is a homeostatic chemokine, constitutively secreted by stromal cells in B cell areas of secondary lymphoid tissues (follicles). CXCR5 is involved in recruitment of circulating naive B cells to follicles and trafficking and homing of B1 B cells. Mice lacking CXCR5 gene display defective primary follicles and germinal centers formation in the spleen and Peyer patches, and lack inguinal lymph nodes.
Additional information: Clone REA215 displays negligible binding to Fc receptors.

Alternative Names

CXCR5, BLR1, CXC-R5, CXCR-5, Gpcr6, MDR15

Technical specifications

  • Antigen: CD185 (CXCR5)
  • Clone: REA215
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CXCR5, BLR1, CXC-R5, CXCR-5, Gpcr6, MDR15
  • Distribution of antigen: B cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, PE-Vio615

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA215
L138D7++
2G8++
SPRCL5++
  • Selected References

    1. Junt, T. et al. (2005) CXCR5-dependent seeding of follicular niches by B and Th cells augments antiviral B cell responses. J. Immunol. 175: 7109-7116
    2. Velaga, S. et al. (2009) Chemokine receptor CXCR5 supports solitary intestinal lymphoid tissue formation, B cell homing, and induction of intestinal IgA responses. J. Immunol. 182: 2610-2619
Product options: 4

Product information

Size
Order no.
Price

CD185 (CXCR5)-PE, mouse

  • REA215
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-103-114
EUR 27,00 

CD185 (CXCR5)-PE, mouse

  • REA215
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-102-288
EUR 80,00 

CD185 (CXCR5)-PE-Vio615, mouse

  • REA215
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-656
EUR 67,00 

CD185 (CXCR5)-PE-Vio615, mouse

  • REA215
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-612
EUR 100,00