CD184 (CXCR4) antibodies, mouse

CD184 (CXCR4) antibodies, mouse

Clone: REA107 | Dilution: 1:50
Clone REA107 reacts with CD184, a receptor for C-X-C chemokine CXCL12/SDF-1. CD184 is a seven membrane spanning receptor and belongs to the family of G-protein coupled chemokine receptors. Expression of CD184 is found in tissues including spleen, thymus, bone marrow, lymph nodes, and at lower levels in small intestine, kidney, and stomach. Diverse functions of CD184 include promoting survival and proliferation of hematopoietic stem cells, inducing apoptosis in T cells, regulating osteoclastogenesis, B cell development, vascularization of the gastrointestinal tract, germ cell migration/survival, and cerebellar development. CD184 also serves as a co-receptor for entry into target cells by HIV-1 X4 and interacts with extracellular ubiquitin.
Additional information: Clone REA107 displays negligible binding to Fc receptors.

Alternative Names

CXCR4, Cmkar4, LESTR, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo, Fusin, SDF-1 receptor

Technical specifications

  • Antigen: CD184 (CXCR4)
  • Clone: REA107
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CXCR4, Cmkar4, LESTR, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo, Fusin, SDF-1 receptor
  • Entrez Gene ID: 12767
  • Molecular mass of antigen [kDa]: 40
  • Distribution of antigen: B cells, T cells, hematopoietic stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE

Specificity

In order to compare the epitope specificity of REAfinity Clone REA107 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA107
L276F12-
2B11++
  • Selected references

    1. Drummond, RA. et al. (2014) Cutting edge: Failure of antigen-specific CD4+ T cell recruitment to the kidney during systemic candidiasis. J. Immunol. 193: 5381-5385
    2. Förster, R. et al. (1998) Intracellular and surface expression of the HIV-1 coreceptor CXCR4/Fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J. Immunol. 160: 1522-1531
    3. Tachibana, K. et al. (1998) The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract. Nature 393: 591-594
    4. Zou Y.-R. et al. (1998) Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development. Nature 393: 595-599
    5. Wegner, S. A. et al. (1998) Genomic organization and functional characterization of the chemokine receptor CXCR4, a major entry co-receptor for human immunodeficiency virus type 1. J. Biol. Chem. 273(8): 4754-4760
Product options: 1
130-118-682

CD184 (CXCR4)-PE, mouse

30 µg in 200 µL (1)
EUR 80,00