CD16 antibodies, human

CD16 antibodies, human

Clone: REA423 | Dilution: 1:50
Clone REA423 recognizes the human CD16 antigen, a single-pass type I membrane protein which is also known as low affinity immunoglobulin gamma Fc region receptor III (FcRIII). CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils. The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone REA423 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes.
Additional information: Clone REA423 displays negligible binding to Fc receptors.

Alternative Names

FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII

Technical specifications

  • Antigen: CD16
  • Clone: REA423
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII
  • Entrez Gene ID: 2214, 2215
  • Molecular mass of antigen [kDa]: 48
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    , capuchin monkey,
    chimpanzee (
    Pan troglodytes
    )
    ,
    common marmoset (
    Callithrix jacchus
    )
    ,
    cotton-top tamarin (
    Saguinus oedipus
    )
    ,
    olive baboon (
    Papio anubis
    )
    ,
    pigtail monkey (
    Macaca nemestrina
    )
    ,
    squirrel monkey (
    Saimiri sciureus
    )
  • Distribution of antigen: NK cells, monocytes, granulocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin, VioBright 515, PE-Vio615
  • Selected References

    1. Romee, R. et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood 121(18): 3599-3608
    2. Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170(2): 481-497
    3. Ziegler-Heitbrock, L. (2007)
      The CD14
      +
      CD16
      +
      blood monocytes: their role in infection and inflammation.
      J. Leukoc. Biol. 584(592): 584-592
    4. Veluchamy, J. et al. (2017)
      In vivo
      efficacy of umbilical cord blood stem cell-derived NK cells in the treatment of metastatic colorectal cancer.
      Front Immunol 8: 87

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA423
3G8++
eBioCB16++
B73.1++
CLB-Fc-gran1/5D2++
Product options: 22

Product information

Size
Order no.
Price

CD16-FITC, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-954
EUR 70,00 

CD16-FITC, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-392
EUR 175,00 

CD16-PE, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-955
EUR 95,00 

CD16-PE, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-393
EUR 235,00 

CD16-APC, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-951
EUR 95,00 

CD16-APC, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-389
EUR 235,00 

CD16-VioBlue, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-958
EUR 115,00 

CD16-VioBlue, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-396
EUR 275,00 

CD16-VioGreen, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-959
EUR 115,00 

CD16-VioGreen, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-397
EUR 275,00 

CD16-PE-Vio770, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-956
EUR 120,00 

CD16-PE-Vio770, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-394
EUR 290,00 

CD16-APC-Vio770, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-952
EUR 120,00 

CD16-APC-Vio770, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-390
EUR 290,00 

CD16-PerCP-Vio700, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-957
EUR 120,00 

CD16-PerCP-Vio700, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-395
EUR 290,00 

CD16-Biotin, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-953
EUR 70,00 

CD16-Biotin, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-391
EUR 175,00 

CD16-VioBright 515, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-119-706
EUR 150,00 

CD16-VioBright 515, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-119-616
EUR 360,00 

CD16-PE-Vio615, human

  • REA423
  • 1:50
  • For research use only
for 30
tests
(1)
130-119-996
EUR 120,00 

CD16-PE-Vio615, human

  • REA423
  • 1:50
  • For research use only
for 100
tests
(1)
130-119-995
EUR 290,00