CD154 antibodies, mouse

CD154 antibodies, mouse

Clone: REA785 | Dilution: 1:50
Clone REA785 specifically recognizes the mouse CD154 antigen, a 39 kDa transmembrane glycoprotein also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4
+
T cells and plays an important role as a costimulatory molecule in T cell/antigen–presenting cell interactions through ligation of CD40. Due to its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4
+
T cells. Adding a CD40-blocking antibody during the stimulation of cell suspensions prevents the down-regulation of CD154. Blocking of CD40 is not required for intracellular detection of CD154 expression or if a pure population of enriched T cells is used.
Additional information: Clone REA785 displays negligible binding to Fc receptors.

Alternative Names

CD40LG, CD40L, HIGM1, IGM, IMD3, Ly-62, T-BAM, TRAP, TNFSF5, gp39

Technical specifications

  • Antigen: CD154
  • Clone: REA785
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CD40LG, CD40L, HIGM1, IGM, IMD3, Ly-62, T-BAM, TRAP, TNFSF5, gp39
  • Entrez Gene ID: 21947
  • Molecular mass of antigen [kDa]: 29
  • Distribution of antigen: basophils, dendritic cells, lymphocytes, macrophages, mast cells, monocytes, NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, VioBright 515, Biotin
Product options: 8

Product information

Size
Order no.
Price

CD154-PE, mouse

  • REA785
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-111-360
EUR 57,00 

CD154-PE, mouse

  • REA785
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-111-172
EUR 224,00 

CD154-APC, mouse

  • REA785
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-111-361
EUR 83,00 

CD154-APC, mouse

  • REA785
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-111-173
EUR 259,00 

CD154-VioBright 515, mouse

  • REA785
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-111-362
EUR 107,00 

CD154-VioBright 515, mouse

  • REA785
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-111-174
EUR 398,00 

CD154-Biotin, mouse

  • REA785
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-111-359
EUR 32,00 

CD154-Biotin, mouse

  • REA785
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-111-171
EUR 147,00 

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