CD14 antibodies, human

CD14 antibodies, human

Clone: REA599 | Dilution: 1:50
Clone REA599 recognizes the human CD14 antigen. CD14 is part of the functional heteromeric LPS receptor complex comprised of at least CD14, TLR4, and MD-2. It up-regulates cell surface molecules, including adhesion molecules. CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other bodily fluids, and in various tissues such as lymph nodes and spleen. CD14 is weakly expressed on subpopulations of human neutrophils and myeloid dendritic cells.
Additional information: Clone REA599 displays negligible binding to Fc receptors.

Alternative Names

LPS R

Technical specifications

  • Antigen: CD14
  • Clone: REA599
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: LPS R
  • Entrez Gene ID: 929
  • Molecular mass of antigen [kDa]: 37
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    , dog, goat, cat, rabbit, mink, bovine, pig, sheep
  • Distribution of antigen: monocytes, macrophages, spleen
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA599
MφP9-
M5E2++
61D3++
TÜK4++
63D3-
  • Selected References

    1. Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press
    2. Wilson, A. D. et al. (1995)
      Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate
      Saguinus oedipus oedipus
      (cotton top tamarin).
      J. Immunol. Methods 178: 195-200
    3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    4. Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8(6): e66898
    5. Paccou, J. et al. (2013)
      Determination and modulation of total and surface calcium-sensing receptor expression in monocytes
      in vivo
      and
      in vitro
      .
      PLoS One 8(10): e74800
Product options: 20

Product information

Size
Order no.
Price

CD14-FITC, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-576
EUR 70,00 

CD14-FITC, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-518
EUR 175,00 

CD14-PE, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-577
EUR 95,00 

CD14-PE, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-519
EUR 235,00 

CD14-APC, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-578
EUR 95,00 

CD14-APC, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-520
EUR 235,00 

CD14-VioBlue, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-582
EUR 115,00 

CD14-VioBlue, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-524
EUR 275,00 

CD14-VioGreen, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-583
EUR 115,00 

CD14-VioGreen, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-525
EUR 275,00 

CD14-PE-Vio615, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-584
EUR 120,00 

CD14-PE-Vio615, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-526
EUR 290,00 

CD14-PE-Vio770, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-579
EUR 120,00 

CD14-PE-Vio770, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-521
EUR 290,00 

CD14-APC-Vio770, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-580
EUR 120,00 

CD14-APC-Vio770, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-522
EUR 290,00 

CD14-PerCP-Vio700, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-581
EUR 120,00 

CD14-PerCP-Vio700, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-523
EUR 290,00 

CD14-Biotin, human

  • REA599
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-575
EUR 70,00 

CD14-Biotin, human

  • REA599
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-517
EUR 175,00