CD133/2 (293C3) antibodies, human

CD133/2 (293C3) antibodies, human

Clone: 293C3 | Dilution: 1:50
CD133 is a novel 5-transmembrane cell surface antigen with a molecular weight of 117 kDa. CD133/2 (293C3) antibodies recognize epitope 2 of the human CD133 antigen (CD133/2). In the hematopoietic system, CD133 expression is restricted to a subset of CD34
bright
stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood. Additionally, CD133 is expressed by a small portion of CD34
cells in these tissues. The CD34
+
CD133
+
cell population, which includes CD34
+
CD38
cells, was shown to be capable of repopulating NOD/SCID mice. Recently, CD133 has also been found to be expressed on circulating endothelial progenitor cells, tissue-specific stem cells, cancer stem cells from tumor tissues, fetal neural stem cells, and ES and iPS cell–derived cells as well as on other tissue-specific stem cells, such as renal, prostate, and corneal stem cells. The putative murine homologue, prominin, which is expressed on neuroepithelial and epithelial mouse cells, was dentified.

Alternative names

PROM1, AC133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4

Technical specifications

  • Antigen: CD133/2 (293C3)
  • Clone: 293C3
  • Isotype: mouse IgG2bκ
  • Isotype control: Mouse IgG2b – isotype control antibodies
  • Alternative names of antigen: PROM1, AC133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
  • Entrez Gene ID: 8842
  • Molecular mass of antigen [kDa]: 117
  • Distribution of antigen: brain, endothelial cells, epithelial cells, heart, hematopoietic stem cells, kidney, liver, lung, pancreas, placenta, ES and iPS cells, red blood cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, Biotin, PE-Vio615
  • Selected references

    1. Cox, J. L. et al. (2012) Elevating SOX2 levels deleteriously affects the growth of medulloblastoma and glioblastoma cells. PLoS One 7(8): e44087
    2. Donnenberg, A. D. et al. (2013) KIT (CD117) expression in a subset of non-small cell lung carcinoma (NSCLC) patients. PLoS One 7(12): e52885
    3. Balasubramanian, P. et al. (2013)
      AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15
      +
      /CalB
      +
      leukocytes.
      PLoS One 8(9): e75764
    4. de Wynter, E. A. et al. (1998)
      CD34
      +
      AC133
      +
      cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
      Stem Cells 16: 387-396
    5. Kang, T. W. et al. (2014) Growth arrest and forced differentiation of human primary glioblastoma multiforme by a novel small molecule. Sci. Rep. 4: 5546
    6. Hawley, R. G. et al. (2006) Hematopoietic stem cells. Meth. Enzymol. 419: 149-179
    7. Golden, J. E. et al. (2012) Human umbilical cord blood cells alter blood and spleen cell populations after stroke. Transl Stroke Res 3(4): 491-499
    8. Wang, L. et al. (2013) Enrichment of prostate cancer stem-like cells from human prostate cancer cell lines by culture in serum-free medium and chemoradiotherapy. Int. J. Biol. Sci. 9(5): 472-479
    9. Miraglia, S. et al. (1997) A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning. Blood 90(12): 5013-5021
    10. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
    11. Gallacher, L. et al. (2000)
      Isolation and characterization of human CD34
      Lin
      and CD34
      +
      Lin
      hematopoietic stem cells using cell surface markers AC133 and CD7.
      Blood 95(ARVO Annual Meeting Abstract): 2813-2820
    12. Peichev, M. et al. (2000)
      Expression of VEGFR-2 and AC133 by circulating human CD34
      +
      cells identifies a population of functional endothelial precursors.
      Blood 95: 952-958
    13. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
    14. Weigmann, A. et al. (1997) Prominin, a novel microvilli-specific polytopic membrane proteine of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells. Proc. Natl. Acad. Sci. U.S.A. 94: 12425-12430
    15. Pfenninger, C. V. et al. (2007) CD133 is not present on neurogenic astrocytes in the adult subventricular zone, but on embryonic neural stem cells, ependymal cells, and glioblastoma cells. Cancer Res. 67(12): 5727-5736
    16. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
    17. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
    18. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
    19. Walton, R. M. et al. (2013)
      Postnatal neural precursor cell regions in the rostral subventricular zone, hippocampal subgranular zone and cerebellum of the dog (
      Canis lupus familiaris
      ).
      Histochem. Cell Biol. 139(3): 415-429
    20. Thill, M. et al. (2004)
      Identification of a population of CD133
      +
      precursor cells in the stroma of human cornea.
      Invest. Ophthalmol. Vis. Sci. 45: 3519
    21. Bühring, H. J. et al. (1991) The product of the proto-oncogene c-kit (P145c-kit) is a human bone marrow surface antigen of hematopoietic precursor cells which is expressed on a subset of acute non-lymphoblastic cells. Leukemia 5: 854-860
    22. Kurian, L. et al. (2013) Conversion of human fibroblasts to angioblast-like progenitor cells. Nat. Methods 10(1): 77-83
  • Brochures and posters

Product options: 12
130-113-750

CD133/2 (293C3)-VioBright FITC, human

for 30
tests
(1)
EUR 211,00 
130-113-188

CD133/2 (293C3)-VioBright FITC, human

for 100
tests
(1)
EUR 430,00 
130-113-748

CD133/2 (293C3)-PE, human

for 30
tests
(1)
EUR 160,00 
130-113-186

CD133/2 (293C3)-PE, human

for 100
tests
(1)
EUR 424,00 
130-113-746

CD133/2 (293C3)-APC, human

for 30
tests
(1)
EUR 211,00 
130-113-184

CD133/2 (293C3)-APC, human

for 100
tests
(1)
EUR 444,00 
130-113-749

CD133/2 (293C3)-PE-Vio770, human

for 30
tests
(1)
EUR 211,00 
130-113-187

CD133/2 (293C3)-PE-Vio770, human

for 100
tests
(1)
EUR 444,00 
130-113-747

CD133/2 (293C3)-Biotin, human

for 30
tests
(1)
EUR 125,00 
130-113-185

CD133/2 (293C3)-Biotin, human

for 100
tests
(1)
EUR 342,00 
130-119-701

CD133/2 (293C3)-PE-Vio615, human

for 30
tests
(1)
EUR 218,00 
130-119-611

CD133/2 (293C3)-PE-Vio615, human

for 100
tests
(1)
EUR 459,00