CD123 antibodies, human

CD123 antibodies, human

Clone: REA918 | Dilution: 1:50
Clone REA918 recognizes the human CD123 antigen, a type I transmembrane glycoprotein, which is also known as interleukin 3 (IL-3) receptor α-chain and is the primary low-affinity subunit of the IL-3 receptor. CD123 associates with CD131, the common β-chain of the IL-3, IL-5, and GM-CSF receptor, to form the high-affinity IL-3 receptor. The IL-3 receptor is involved in cell signaling for cell growth and differentiation. In peripheral blood, the CD123 antigen is expressed at high levels only on plasmacytoid dendritic cells and basophilic granulocytes but at low levels also on monocytes, eosinophilic granulocytes, myeloid dendritic cells, and subsets of hematopoietic progenitor cells.
Additional information: Clone REA918 displays negligible binding to Fc receptors.

Alternative Names

IL3RA, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, IL-3Rα

Technical specifications

  • Antigen: CD123
  • Clone: REA918
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL3RA, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, IL-3Rα
  • Entrez Gene ID: 3563
  • Molecular mass of antigen [kDa]: 41
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
  • Distribution of antigen: dendritic cells, granulocytes, mast cells, megakaryocytes, monocytes, basophils, eosinophils, hematopoietic stem cells, myeloid cells, plasma cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin, VioBright 667

Specificity

In order to compare the epitope specificity of REAfinity Clone REA918 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA918
AC145++
6H6++
7G3++
32703+
  • Selected references

    1. Miyajima, A. et al. (1993) Receptors for granulocyte-macrophage colony-stimulating factor, interleukin-3, and interleukin-5. Blood 82(7): 1960-1974
    2. Chen, J. et al. (2009) A new isoform of interleukin-3 receptor {alpha} with novel differentiation activity and high affinity binding mode. J. Biol. Chem. 284(9): 5763-5773
    3. Choi, K.-D. et al. (2011) Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells. Nat. Protoc. 6(3): 296-313
Product options: 22
130-115-356

CD123-FITC, human

for 30
tests
(1)
EUR 90,00 
130-115-263

CD123-FITC, human

for 100
tests
(1)
EUR 220,00 
130-115-357

CD123-PE, human

for 30
tests
(1)
EUR 110,00 
130-115-264

CD123-PE, human

for 100
tests
(1)
EUR 265,00 
130-115-358

CD123-APC, human

for 30
tests
(1)
EUR 110,00 
130-115-265

CD123-APC, human

for 100
tests
(1)
EUR 265,00 
130-115-363

CD123-VioBlue, human

for 30
tests
(1)
EUR 115,00 
130-115-270

CD123-VioBlue, human

for 100
tests
(1)
EUR 275,00 
130-115-364

CD123-VioGreen, human

for 30
tests
(1)
EUR 115,00 
130-115-271

CD123-VioGreen, human

for 100
tests
(1)
EUR 275,00 
130-115-365

CD123-PE-Vio615, human

for 30
tests
(1)
EUR 120,00 
130-115-272

CD123-PE-Vio615, human

for 100
tests
(1)
EUR 290,00 
130-115-359

CD123-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-115-266

CD123-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-115-360

CD123-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-115-267

CD123-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-115-361

CD123-PerCP-Vio700, human

for 30
tests
(1)
EUR 120,00 
130-115-268

CD123-PerCP-Vio700, human

for 100
tests
(1)
EUR 290,00 
130-115-355

CD123-Biotin, human

for 30
tests
(1)
EUR 90,00 
130-115-262

CD123-Biotin, human

for 100
tests
(1)
EUR 220,00 
130-118-374

CD123-VioBright 667, human

for 30
tests
(1)
EUR 150,00 
130-118-220

CD123-VioBright 667, human

for 100
tests
(1)
EUR 360,00