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Human peripheral blood mononuclear cells (PBMCs) were spiked with human umbilical vein endothelial cells (HUVECs) and stained with CD105 antibodies as well as with CD45 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.