Anti-TCRγ/δ antibodies, mouse

Anti-TCRγ/δ antibodies, mouse

Clone: REA633 | Dilution: 1:50
Clone REA633 recognizes the mouse TCRγ/δ antigen. The T cell receptor (TCR) is a heterodimeric glycoprotein associated with the CD3 antigen. It consists of an α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit. The γ/δ T cell receptor is present on a subset of T lymphocytes in peripheral blood. TCRγ/δ is involved in the antigen recognition of tumor-associated antigens or bacterial antigens presented by MHC class I molecules.
Additional information: Clone REA633 displays negligible binding to Fc receptors.

Alternative names

TCRgd

Technical specifications

  • Antigen: TCRγ/δ
  • Clone: REA633
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: TCRgd
  • Entrez Gene ID: 110066, 110067
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PerCP-Vio700, PE, PE-Vio770

Specificity

In order to compare the epitope specificity of REAfinity Clone REA633 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA633
GL3++
UC7-13D5++
  • Selected references

    1. Schmolka, N. et al. (2013) Epigenetic and transcriptional signatures of stable versus plastic differentiation of proinflammatory γδ T cell subsets. Nat. Immunol. 14(10): 1093-1100
    2. Bonneville, M. et al. (2010) Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat. Rev. Immunol. 10(7): 467-478
    3. Wiest, D. L. (2016) Development of γδ T cells, the special-force soldiers of the immune system. Methods Mol. Biol. 1323: 23-32
Product options: 4
130-117-777

Anti-TCRγ/δ-PerCP-Vio700, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-117-665

Anti-TCRγ/δ-PerCP-Vio700, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-122-222

Anti-TCRγ/δ-PE, mouse

30 µg in 200 µL (1)
EUR 80,00 
130-123-290

Anti-TCRγ/δ-PE-Vio770, mouse

30 µg in 200 µL (1)
EUR 100,00