Anti-Notch1 antibodies, human

Anti-Notch1 antibodies, human

Clone: REA849 | Dilution: 1:50
Clone REA849 recognizes the human neurogenic locus notch homolog protein 1 (Notch1) antigen, a 300 kDa single-pass type I membrane protein also known as translocation-associated notch protein (TAN-1). Following cleavage in the Golgi, notch forms a heterodimer composed of a 180–200 kDa disulfide-linked extracellular domain (Notch extracellular truncation (NEXT)), and a 120 kDa membrane-bound intracellular portion (Notch intracellular domain (NICD)). Upon ligand binding the latter is cleaved to form the notch intracellular domain transcription factor. Notch signaling controls cell fate decisions and is involved in multiple cellular differentiation steps during embryonic development and in adult organisms. In the immune system, Notch1 plays a non-redundant role for thymic T cell development. Notch1 is also expressed by peripheral T cells and upregulated upon T cell activation and also found on bone marrow hematopoietic stem cells, NK cells, B cells, and monocytes .
Additional information: Clone REA849 displays negligible binding to Fc receptors.

Alternative names

Notch 1, TAN-1, Motch A, mT14, p300, hN1

Technical specifications

  • Antigen: Notch1
  • Clone: REA849
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Notch 1, TAN-1, Motch A, mT14, p300, hN1
  • Entrez Gene ID: 18128
  • Molecular mass of antigen [kDa]: 269
  • Distribution of antigen: T cells, bone marrow, hematopoietic stem cells, NK cells, B cells, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBright 515, Biotin
  • Selected references

    1. Gray, G. E. et al. (1999) Human ligands of the Notch receptor. Am. J. Pathol. 154(3): 785-794
    2. Weng, A. P. et al. (2004) Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia. Science 306(5694): 269-271
    3. Arruga, F. et al. (2014) Functional impact of NOTCH1 mutations in chronic lymphocytic leukemia. Leukemia 28(5): 1060-1070

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