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Heart tissue from P1 Wistar rats was dissociated using the Neonatal Heart Dissociation Kit and the gentleMACS™ Dissociator. Neonatal cardiomyocytes were then fixed, permeabilized, and stained with Anti-MLC2a antibodies or with the corresponding REA Control (I) antibodies (left images). Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.