Anti-MHC Class II (I-Ab) antibodies, mouse

Anti-MHC Class II (I-Ab) antibodies, mouse

Clone: REA528 | Dilution: 1:50
Clone REA528 recognizes the mouse MHC class II alloantigen I-Ab of H-2b bearing mouse strains. It also cross-reacts with the H-2k and H-2u haplotypes, but reactivity to other haplotypes like, for example, d, f, g7, p, q, r, and s has not been observed. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes, macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.
Additional information: Clone REA528 displays negligible binding to Fc receptors.

Alternative Names

MHC class II IAb

Technical specifications

  • Antigen: MHC Class II (I-Ab)
  • Clone: REA528
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: MHC class II IAb
  • Entrez Gene ID: 14961
  • Molecular mass of antigen [kDa]: 27
  • Distribution of antigen: B cells, dendritic cells, hematopoietic stem cells, macrophages, monocytes, thymic epithelial cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clonesOverlap in epitope recognition with REA528
25-9-17+
AF6-120.1++
KH74-
Product options: 2

Product information

Size
Order no.
Price

Anti-MHC Class II (I-Ab)-PE, mouse

  • REA528
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-117-541
EUR 72,00 

Anti-MHC Class II (I-Ab)-PE, mouse

  • REA528
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-117-392
EUR 207,00 

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