Anti-Ly-6G antibodies, mouse

Anti-Ly-6G antibodies, mouse

Clone: REA526 | Dilution: 1:10
Clone REA526 recognizes the mouse lymphocyte antigen 6G (Ly-6G) antigen, a 21–25 kDa glycosylphosphatidylinositol (GPI)-linked protein which is highly expressed on neutrophilic granulocytes. Expression greatly increases during granulocyte maturation. To a lower extent, Ly-6G is also expressed during the development of monocytes. In mouse bone marrow samples, Ly-6G is primarily detected on granulocytes, but not on lymphocytes or erythrocytes. Ligation of Ly-6G in animals with an underlying background inflammation (induced by TNF-α) leads to profound cardiovascular and respiratory responses, including severe breathing difficulties, leukocyte and platelet aggregation, and coagulation, leading to microvessel obstruction and subsequent death.
Additional information: Clone REA526 displays negligible binding to Fc receptors.

Alternative names

Ly-6G.1, Ly6g, Gr-1

Technical specifications

  • Antigen: Ly-6G
  • Clone: REA526
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Ly-6G.1, Ly6g, Gr-1
  • Entrez Gene ID: 546644
  • Molecular mass of antigen [kDa]: 10
  • Distribution of antigen: granulocytes, macrophages, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, PE-Vio770

Specificity

In order to compare the epitope specificity of REAfinity Clone REA526 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA526
REA810-
1A8++
RB6-8C5-
  • Selected references

    1. Carr, K. D. et al. (2011) Specific depletion reveals a novel role for neutrophil-mediated protection in the liver during Listeria monocytogenes infection. Eur. J. Immunol. 41(9): 2666-2676
    2. Tanaka, Y. et al. (2012) Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock. J. Leukoc. Biol. 91(3): 485-494
    3. Fleming, T. J. et al. (1993) Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J. Immunol. 150(12): 5379-5390
Product options: 4
130-107-975

Anti-Ly-6G-PE, mouse

9 µg in 300 µL (1)
EUR 39,00 
130-107-913

Anti-Ly-6G-PE, mouse

30 µg in 1 mL (1)
EUR 60,00 
130-107-977

Anti-Ly-6G-PE-Vio770, mouse

9 µg in 300 µL (1)
EUR 67,00 
130-107-915

Anti-Ly-6G-PE-Vio770, mouse

30 µg in 1 mL (1)
EUR 100,00