Anti-Ly-6B.2 antibodies, mouse

Anti-Ly-6B.2 antibodies, mouse

Clone: REA115 | Dilution: 1:10
Clone REA115 recognizes Ly-6B.2, a member of the lymphocyte antigen 6 (Ly-6) superfamily and is located on chromosome 15 as the other members of the murine Ly-6 family. The Ly-6 family members are normally GPI-anchored, cysteine-rich cell surface molecules. Expression of Ly-6B.2 antigen has been reported on neutrophils, bone marrow restricted progenitors, monocytes, and is lost during the differentiation of monoytes into tissue resident macrophages or dendritic cell phenotype. High expression of Ly-6B.2 is also associated with inflammatory cell repertoire including monocytes and certain populations of activated macrophages present during infections.
Additional information: Clone REA115 displays negligible binding to Fc receptors.

Alternative names

LY6B

Technical specifications

  • Antigen: Ly-6B.2
  • Clone: REA115
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: LY6B
  • Entrez Gene ID: 110453
  • Distribution of antigen: neutrophils, monocytes, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA115 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA115
7/4++
  • Selected references

    1. Rosas, M. et al. (2010) The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J. Leukoc. Biol. 88: 169-180
    2. Hirsch, S. et al. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4. Immunogenetics 18(3): 229-239
Product options: 13
130-103-318

Anti-Ly-6B.2-FITC, mouse

9 µg in 300 µL (1)
EUR 20,00 
130-102-279

Anti-Ly-6B.2-FITC, mouse

30 µg in 1 mL (1)
EUR 40,00 
130-102-857

Anti-Ly-6B.2-PE, mouse

9 µg in 300 µL (1)
EUR 27,00 
130-102-370

Anti-Ly-6B.2-PE, mouse

30 µg in 1 mL (1)
EUR 80,00 
130-102-856

Anti-Ly-6B.2-APC, mouse

9 µg in 300 µL (1)
EUR 44,00 
130-102-322

Anti-Ly-6B.2-APC, mouse

30 µg in 1 mL (1)
EUR 80,00 
130-105-418

Anti-Ly-6B.2-PE-Vio770, mouse

9 µg in 300 µL (1)
EUR 52,00 
130-105-377

Anti-Ly-6B.2-PE-Vio770, mouse

30 µg in 1 mL (1)
EUR 100,00 
130-106-144

Anti-Ly-6B.2-APC-Vio770, mouse

9 µg in 300 µL (1)
EUR 56,00 
130-106-093

Anti-Ly-6B.2-APC-Vio770, mouse

30 µg in 1 mL (1)
EUR 100,00 
130-112-970

Anti-Ly-6B.2-PerCP-Vio700, mouse

30 µg in 1 mL (1)
EUR 100,00 
130-102-003

Anti-Ly-6B.2-Biotin, mouse

9 µg in 300 µL (1)
EUR 15,00 
130-101-881

Anti-Ly-6B.2-Biotin, mouse

30 µg in 1 mL (1)
EUR 40,00