Anti-Ly-49C/I antibodies, mouse

Anti-Ly-49C/I antibodies, mouse

Clone: REA253 | Dilution: 1:10
Clone REA253 recognizes the mouse lymphocyte antigen 49c (Ly-49C), a type II transmembrane protein which is also known as killer cell lectin-like receptor 3 (Klra3). The REA253 antibody reacts with Ly-49C of BALB, B6, NZB, C57BL, and Ly-49I of B6 mice strains. Ly-49 family members are both activating and inhibitory receptors and are mainly receptors for MHC class I molecules. Ly-49C is a cell surface molecule expressed on a subpopulation of murine natural killer cells and NK1.1 T cells that are involved in the specific rejection of H-2
d
or H-2
f
(hemopoietic histocompatibility determinant 2) bone marrow cell grafts. It transduces a negative signal to NK cells upon recognition of class I antigens. The particular allele of Ly-49C, i.e., C57BL versus BALB, determines to a large extent which class I antigen (H-2K
b
for B6, K
b
and H-2
d
for BALB/c or BALB.B) sends negative signals to Ly-49C
+
NK cells. Clone REA253 blocks the binding of Ly-49C to MHC class I antigens of the b, d, k, and s haplotypes. The epitope recognized by REA253 may be masked on NK cells due to interactions with MHC class I molecules.
Additional information: Clone REA253 displays negligible binding to Fc receptors.

Alternative names

Klra3, Klra9

Technical specifications

  • Antigen: Ly-49C/I
  • Clone: REA253
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Klra3, Klra9
  • Entrez Gene ID: 16634, 16640
  • Molecular mass of antigen [kDa]: 31
  • Distribution of antigen: NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, Biotin
  • Selected references

    1. Yu, Y. Y. et al. (1996) The role of Ly49A and 5E6(Ly49C) molecules in hybrid resistance mediated by murine natural killer cells against normal T cell blasts. Immunity 4(1): 67-76
    2. Brennan, J. et al. (1996) Heterogeneity among Ly-49C natural killer (NK) cells: characterization of highly related receptors with differing functions and expression patterns. J. Exp. Med. 184(6): 2085-2090
    3. Stoneman, E. R. et al. (1995) Cloning and characterization of 5E6(Ly-49C), a receptor molecule expressed on a subset of murine natural killer cells. J. Exp. Med. 182(2): 305-313
Product options: 6
9 µg in 300 µL (1)
EUR 27,00 
30 µg in 1 mL (1)
EUR 80,00 
9 µg in 300 µL (1)
EUR 44,00 
30 µg in 1 mL (1)
EUR 80,00 
9 µg in 300 µL (1)
EUR 15,00 
30 µg in 1 mL (1)
EUR 40,00 

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