Anti-LGR5 antibodies, human

Anti-LGR5 antibodies, human

Clone: REA762 | Dilution: 1:50
Clone REA762 recognizes the human leucine-rich repeat-containing G-protein–coupled receptor 5 (LGR5) but not with its close homologues LGR4 or LGR6. LGR5 associates with Wnt-receptors and act as R-spondin receptor thereby playing a central role in the modulation of Wnt/β-catenin signaling in normal and neoplastic stem cells. Initially described as a highly specific marker for stem cells in the small intestine, colon, hair follicle, stomach, and during kidney development, LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5 positive crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression.
Additional information: Clone REA762 displays negligible binding to Fc receptors.

Alternative Names

Fex, GPR49, GPR67, GRP49, HG38

Technical specifications

  • Antigen: LGR5
  • Clone: REA762
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Fex, GPR49, GPR67, GRP49, HG38
  • Entrez Gene ID: 8549
  • Molecular mass of antigen [kDa]: 98
  • Distribution of antigen: cancer stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin
  • Selected References

    1. Barker, N. et al. (2007) Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449(7165): 1003-1007
    2. Barker, N. et al. (2012)
      LGR5
      +
      stem/progenitor cells contribute to nephron formation during kidney development.
      Cell Rep 2: 540-552
    3. Jaks, V. et al. (2008) Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat. Genet. 40: 1291-1299
    4. Barker, N. et al. (2009) Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 457: 608-611
    5. Barker, N. et al. (2010)
      Lgr5
      +
      stem cells drive self-renewal in the stomach and build long-lived gastric units
      in vitro
      .
      Cell Stem Cell 6(1): 25-36
    6. Carmon, K. S. et al. (2011) R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/β-catenin signaling. Proc. Natl. Acad. Sci. U.S.A. 108: 11452-11457
    7. de Lau, W. et al. (2011) Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476(7360): 293-297
    8. Kemper, K. et al. (2012) Monoclonal antibodies against lgr5 identify human colorectal cancer stem cells. Stem Cells 30(11): 2378-2386
    9. Dame, M. K. et al. (2014) Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived. Lab. Invest. 94(2): 222-234
Product options: 12
130-112-508

Anti-LGR5-FITC, human

for 30
tests
(1)
EUR 90,00 
130-112-436

Anti-LGR5-FITC, human

for 100
tests
(1)
EUR 220,00 
130-112-509

Anti-LGR5-PE, human

for 30
tests
(1)
EUR 110,00 
130-112-437

Anti-LGR5-PE, human

for 100
tests
(1)
EUR 265,00 
130-112-510

Anti-LGR5-APC, human

for 30
tests
(1)
EUR 110,00 
130-112-438

Anti-LGR5-APC, human

for 100
tests
(1)
EUR 265,00 
130-112-511

Anti-LGR5-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-112-439

Anti-LGR5-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-112-512

Anti-LGR5-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-112-440

Anti-LGR5-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-112-507

Anti-LGR5-Biotin, human

for 30
tests
(1)
EUR 90,00 
130-112-435

Anti-LGR5-Biotin, human

for 100
tests
(1)
EUR 220,00