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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Naive CD4 + CD62L + T cells were isolated from mouse spleen cells using the CD4 + CD62L + T Cell Isolation Kit, mouse. Cells were stimulated with CD3ε pure – functional grade and CD28 pure – functional grade antibodies in the presence of recombinant mouse IL-2 and mouse IL-4, as well as Anti-IFN-γ pure – functional grade antibodies for three days. Then cells were harvested, washed, and incubated with or without (left images) PMA/ionomycin and brefeldin A. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-3, CD4, and CD154 antibodies. Cells were pre-gated for CD4 + and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris were excluded. |
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