Anti-IL-12R β2 antibodies, human

Anti-IL-12R β2 antibodies, human

Clone: REA333 | Dilution: 1:11
Clone REA333 recognizes the human interleukin 12 (IL-12) receptor subunit beta-2 (IL-12R β2) antigen, a single-pass type I membrane protein which forms the functional high affinity IL-12 receptor together with the IL-12R β1 subunit. While the IL-12R β1 subunit is constitutively expressed, the expression of the IL-12R β2 gene is up-regulated by interferon γ. IL-12R β2 is expressed by Tʜ1 cells and IL-12 signaling through the IL-12 receptor leads to the phosphorylation of STAT4 and continued Tʜ1 differentiation. The IL-12R β2 subunit plays an important role in Tʜ1 cell differentiation, since its absence leads to an abortive Tʜ1 differentiation that has dysfunctional production of Tʜ1 effector molecules. The up-regulation of IL-12R β2 is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense.
Additional information: Clone REA333 displays negligible binding to Fc receptors.

Alternative names

IL12RB2

Technical specifications

  • Antigen: IL-12R β2
  • Clone: REA333
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL12RB2
  • Entrez Gene ID: 3595
  • Molecular mass of antigen [kDa]: 95
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, Biotin

  • Figure 1

    Human CD4
    +
    T cells were either left unstimulated (left images) or stimulated with CD3/CD28/Anti-IL-4 antibodies and IL-2/IL-12 for 6 days. Cells were then restimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 1 hour and stained with Anti-IL-12R β2 antibodies as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant
    ®
    Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  • Selected references

    1. Nishikomori, R. et al. (2002) Activated STAT4 has an essential role in Tʜ1 differentiation and proliferation that is independent of its role in the maintenance of IL-12R beta 2 chain expression and signaling. J. Immunol. 169(8): 4388-4398
    2. Salomon, D. R. et al. (2017) H3K27 methylation dynamics during CD4 T cell activation: regulation of JAK/STAT and IL12RB2 Eexpression by JMJD3. J. Immunol. 199(9): 3158-3175
    3. Yin, Z. et al. (2000) Dominance of IL-12 over IL-4 in gamma delta T cell differentiation leads to default production of IFN-gamma: failure to down-regulate IL-12 receptor beta 2-chain expression. J. Immunol. 164(6): 3056-3064
    4. Presky, D. H. et al. (1996) A functional interleukin 12 receptor complex is composed of two beta-type cytokine receptor subunits. Proc. Natl. Acad. Sci. U.S.A. 93(24): 14002-14007
Product options: 4
130-105-036

Anti-IL-12R β2-APC, human

for 30
tests
(1)
EUR 110,00 
130-104-984

Anti-IL-12R β2-APC, human

for 100
tests
(1)
EUR 265,00 
130-105-034

Anti-IL-12R β2-Biotin, human

for 30
tests
(1)
EUR 90,00 
130-104-982

Anti-IL-12R β2-Biotin, human

for 100
tests
(1)
EUR 220,00 
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