Anti-IL-12R β2 antibodies, human

Technical specifications

• Antigen: IL-12R β2
• Clone: REA333
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: IL12RB2
• Entrez Gene ID: 3595
• Molecular mass of antigen [kDa]: 95
• Distribution of antigen: T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC, Biotin

• Figure 1

  Human CD4 + T cells were either left unstimulated (left images) or stimulated with CD3/CD28/Anti-IL-4 antibodies and IL-2/IL-12 for 6 days. Cells were then restimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 1 hour and stained with Anti-IL-12R β2 antibodies as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

• Selected references

  1. Nishikomori, R. et al. (2002) Activated STAT4 has an essential role in Tʜ1 differentiation and proliferation that is independent of its role in the maintenance of IL-12R beta 2 chain expression and signaling. J. Immunol. 169(8): 4388-4398
  2. Salomon, D. R. et al. (2017) H3K27 methylation dynamics during CD4 T cell activation: regulation of JAK/STAT and IL12RB2 Eexpression by JMJD3. J. Immunol. 199(9): 3158-3175
  3. Yin, Z. et al. (2000) Dominance of IL-12 over IL-4 in gamma delta T cell differentiation leads to default production of IFN-gamma: failure to down-regulate IL-12 receptor beta 2-chain expression. J. Immunol. 164(6): 3056-3064
  4. Presky, D. H. et al. (1996) A functional interleukin 12 receptor complex is composed of two beta-type cytokine receptor subunits. Proc. Natl. Acad. Sci. U.S.A. 93(24): 14002-14007