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Technical support - Clinical products
Splenocytes from C57BL/6 mice were either left unstimulated (left images) or stimulated with 20 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 2 hours, followed by an incubation with 1 µg/mL brefeldin A for 4 hours. Cells were then fixed, permeabilized, and stained with Anti-IL-10 antibodies as well as with CD4 and CD154 antibodies and analyzed by flow cytometry using the MACSQuant®
cells were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.