Anti-HLA-DQ antibodies, human

Anti-HLA-DQ antibodies, human

Clone: REA303 | Dilution: 1:11
Clone REA303 recognizes HLA-DQ, an αβ heterodimer of the MHC class II type. Expressed on the surface of most nucleated cells, MHC class II molecules play an important role in the immune system. They are essential in the defense against infection and are a main consideration in transplantation medicine. In addition to presenting antigenic peptides from predominantly extracellular sources to CD4
+
T cells, MHC class II molecules also mediate the thymic selection of T helper cells. MHC class II molecules consist of an α and β chain and are transported to endosomal-lysosomal compartments by the invariant chain. In humans, MHC class II molecules are encoded by three different loci, HLA-DR, -DQ, and -DP, which display 70% similarity to each other. Both chains of HLA-DQ are polymorphic. Despite the essential function of MHC class II molecules in immune defense against pathogens, some alleles are frequently linked to immune diseases. Two autoimmune diseases in which HLA-DQ is involved are coeliac disease and diabetes mellitus type 1.
Additional information: Clone REA303 displays negligible binding to Fc receptor

Alternative Names

HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2

Technical specifications

  • Antigen: HLA-DQ
  • Clone: REA303
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2
  • Entrez Gene ID: 3117, 3118, 3119, 3120, 3121
  • Molecular mass of antigen [kDa]: 26
  • Distribution of antigen: B cells, dendritic cells, macrophages, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA303 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA 303
SK10-
HLADQ1-
Tü169++
REA332-
AC122-
REA805-
  • Selected references

    1. van Lith, M. et al. (2010) HLA-DP, HLA-DQ, and HLA-DR have different requirements for invariant chain and HLA-DM. J. Biol. Chem. 285(52): 40800-40808
    2. Jones, E. Y. et al. (2006) MHC class II proteins and disease: a structural perspective. Nat. Rev. Immunol. 6(4): 271-282
    3. Klitz, W. et al. (2003) New HLA haplotype frequency reference standards: high-resolution and large sample typing of HLA DR-DQ haplotypes in a sample of European Americans. Tissue Antigens 62(4): 296-307
Product options: 12
130-104-677

Anti-HLA-DQ-FITC, human

for 30
tests
(1)
EUR 90,00 
130-104-605

Anti-HLA-DQ-FITC, human

for 100
tests
(1)
EUR 220,00 
130-104-545

Anti-HLA-DQ-PE, human

for 30
tests
(1)
EUR 110,00 
130-104-496

Anti-HLA-DQ-PE, human

for 100
tests
(1)
EUR 265,00 
130-104-546

Anti-HLA-DQ-APC, human

for 30
tests
(1)
EUR 110,00 
130-104-497

Anti-HLA-DQ-APC, human

for 100
tests
(1)
EUR 265,00 
130-104-547

Anti-HLA-DQ-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-104-498

Anti-HLA-DQ-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-104-548

Anti-HLA-DQ-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-104-499

Anti-HLA-DQ-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-104-544

Anti-HLA-DQ-Biotin, human

for 30
tests
(1)
EUR 90,00 
130-104-495

Anti-HLA-DQ-Biotin, human

for 100
tests
(1)
EUR 220,00