Anti-Gr-1 antibodies, mouse

Anti-Gr-1 antibodies, mouse

Clone: REA810 | Dilution: 1:50
Clone REA810 recognizes the mouse granulocyte-differentiation antigen-1 (Gr-1) antigen, also known as Ly-6G, a 21–25 kDa, GPI-anchored cell surface protein. Cross-reactivity of Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G. Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
Additional information: Clone REA810 displays negligible binding to Fc receptors.

Alternative Names

Ly-6G, Ly-6G.1

Technical specifications

  • Antigen: Gr-1
  • Clone: REA810
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Ly-6G, Ly-6G.1
  • Entrez Gene ID: 546644
  • Molecular mass of antigen [kDa]: 10
  • Distribution of antigen: granulocytes, monocytes, bone marrow
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700

Specificity

In order to compare the epitope specificity of REAfinity Clone REA810 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA810
REA526++
1A8++
RB6-8C5++
  • Selected references

    1. Fleming, T. J. et al. (1993) Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J. Immunol. 150(12): 5379-5390
    2. Fleming, T. J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J. Immunol. 151: 2399-2408
    3. Nagendra, S. and Schlueter, A. J. (2004) Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 58: 195-200
Product options: 17
130-112-311

Anti-Gr-1-FITC, mouse

30 µg in 200 µL (1)
EUR 35,00 
130-112-153

Anti-Gr-1-FITC, mouse

150 µg in 1 mL (1)
EUR 100,00 
130-112-306

Anti-Gr-1-PE, mouse

30 µg in 200 µL (1)
EUR 60,00 
130-112-148

Anti-Gr-1-PE, mouse

150 µg in 1 mL (1)
EUR 175,00 
130-112-307

Anti-Gr-1-APC, mouse

30 µg in 200 µL (1)
EUR 60,00 
130-112-149

Anti-Gr-1-APC, mouse

150 µg in 1 mL (1)
EUR 175,00 
130-112-302

Anti-Gr-1-VioBlue, mouse

30 µg in 200 µL (1)
EUR 95,00 
130-112-144

Anti-Gr-1-VioBlue, mouse

150 µg in 1 mL (1)
EUR 275,00 
130-112-303

Anti-Gr-1-VioGreen, mouse

30 µg in 200 µL (1)
EUR 95,00 
130-112-304

Anti-Gr-1-PE-Vio615, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-112-146

Anti-Gr-1-PE-Vio615, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-112-308

Anti-Gr-1-PE-Vio770, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-112-150

Anti-Gr-1-PE-Vio770, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-112-309

Anti-Gr-1-APC-Vio770, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-112-151

Anti-Gr-1-APC-Vio770, mouse

150 µg in 1 mL (1)
EUR 295,00 
130-112-310

Anti-Gr-1-PerCP-Vio700, mouse

30 µg in 200 µL (1)
EUR 100,00 
130-112-152

Anti-Gr-1-PerCP-Vio700, mouse

150 µg in 1 mL (1)
EUR 295,00